Linked Life Data

20196153

Source:http://linkedlifedata.com/resource/pubmed/id/20196153

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
2010-4-14
pubmed:abstractText
The apoferritin protein and apoferritin-Tb(3+) complex were demonstrated to form oligomeric and polymeric self-assemblies in neutral aqueous solutions, based on characterization by using luminescence and UV/Vis spectroscopy, dynamic light scattering, and transmission electron microscopy. Addition of a 20-mer or higher poly(arginine) to the solution resulted in coprecipitation through nanoscale interactions, while biological proteins and other poly(amino acids) rarely yielded precipitates under the conditions employed. The apoferritin-Tb(3+) complex assembly exhibited a particularly long-lived green luminescence in aqueous solution, and its poly(arginine)-selective precipitation behavior was followed by monitoring the changes in luminescence. The poly(arginine)-tagged albumin precipitated selectively and quantitatively, so that the apoferritin-Tb(3+) complex can function as a new luminescent biotool for the sensing of poly(arginine) and its protein conjugates.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1521-3765
pubmed:author
pubmed:issnType
Electronic
pubmed:day
12
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4273-8
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Poly(arginine)-selective coprecipitation properties of self-assembling apoferritin and Its Tb(3+) complex: a new luminescent biotool for sensing of poly(arginine) and its protein conjugates.
pubmed:affiliation
Department of Chemistry, Graduate School of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan. tsukube@sci.osaka-cu.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't