pubmed-article:2019434 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:2019434 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
pubmed-article:2019434 | lifeskim:mentions | umls-concept:C0021585 | lld:lifeskim |
pubmed-article:2019434 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
pubmed-article:2019434 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
pubmed-article:2019434 | lifeskim:mentions | umls-concept:C1511040 | lld:lifeskim |
pubmed-article:2019434 | lifeskim:mentions | umls-concept:C1524063 | lld:lifeskim |
pubmed-article:2019434 | lifeskim:mentions | umls-concept:C0076206 | lld:lifeskim |
pubmed-article:2019434 | pubmed:issue | 5 | lld:pubmed |
pubmed-article:2019434 | pubmed:dateCreated | 1991-5-28 | lld:pubmed |
pubmed-article:2019434 | pubmed:abstractText | The baculovirus expression vector p36C was used to express in cells of the insect Spodoptera frugiperda fragment C of tetanus toxin under the control of the strong polyhedrin promoter. Fragment C was expressed intracellularly at a high level and was soluble, allowing it to be purified by affinity chromatography with monoclonal antibody TT08. Purified fragment C from baculovirus was used to immunize mice and was shown to successfully prevent the symptoms of tetanus following a toxin challenge. The ganglioside-binding properties of baculovirus-derived fragment C were compared with those of intact tetanus toxin and native fragment C and were found to be dissimilar. | lld:pubmed |
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pubmed-article:2019434 | pubmed:language | eng | lld:pubmed |
pubmed-article:2019434 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2019434 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:2019434 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:2019434 | pubmed:month | May | lld:pubmed |
pubmed-article:2019434 | pubmed:issn | 0019-9567 | lld:pubmed |
pubmed-article:2019434 | pubmed:author | pubmed-author:MakoffA JAJ | lld:pubmed |
pubmed-article:2019434 | pubmed:author | pubmed-author:FairweatherN... | lld:pubmed |
pubmed-article:2019434 | pubmed:author | pubmed-author:ChatfieldS... | lld:pubmed |
pubmed-article:2019434 | pubmed:author | pubmed-author:CharlesI GIG | lld:pubmed |
pubmed-article:2019434 | pubmed:author | pubmed-author:RodgersB CBC | lld:pubmed |
pubmed-article:2019434 | pubmed:author | pubmed-author:SlaterD EDE | lld:pubmed |
pubmed-article:2019434 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:2019434 | pubmed:volume | 59 | lld:pubmed |
pubmed-article:2019434 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:2019434 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:2019434 | pubmed:pagination | 1627-32 | lld:pubmed |
pubmed-article:2019434 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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pubmed-article:2019434 | pubmed:year | 1991 | lld:pubmed |
pubmed-article:2019434 | pubmed:articleTitle | Synthesis of tetanus toxin fragment C in insect cells by use of a baculovirus expression system. | lld:pubmed |
pubmed-article:2019434 | pubmed:affiliation | Department of Molecular Biology, Wellcome Research Laboratories, Beckenham, Kent, England. | lld:pubmed |
pubmed-article:2019434 | pubmed:publicationType | Journal Article | lld:pubmed |
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