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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1991-5-28
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pubmed:abstractText |
The baculovirus expression vector p36C was used to express in cells of the insect Spodoptera frugiperda fragment C of tetanus toxin under the control of the strong polyhedrin promoter. Fragment C was expressed intracellularly at a high level and was soluble, allowing it to be purified by affinity chromatography with monoclonal antibody TT08. Purified fragment C from baculovirus was used to immunize mice and was shown to successfully prevent the symptoms of tetanus following a toxin challenge. The ganglioside-binding properties of baculovirus-derived fragment C were compared with those of intact tetanus toxin and native fragment C and were found to be dissimilar.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-1104484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-2105271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-2318526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-2542937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-2643085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-2690015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-2778708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-2914606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-3059993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-3068965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-3312002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-3533452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-3536478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-3553425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-3681984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-3774547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-3785374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-390355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-401808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-40973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-6198285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-6272284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-6296842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-6325875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-64469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-6711309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2019434-964380
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1627-32
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2019434-Animals,
pubmed-meshheading:2019434-Baculoviridae,
pubmed-meshheading:2019434-Base Sequence,
pubmed-meshheading:2019434-Cloning, Molecular,
pubmed-meshheading:2019434-Gangliosides,
pubmed-meshheading:2019434-Genetic Vectors,
pubmed-meshheading:2019434-Mice,
pubmed-meshheading:2019434-Mice, Inbred BALB C,
pubmed-meshheading:2019434-Molecular Sequence Data,
pubmed-meshheading:2019434-Moths,
pubmed-meshheading:2019434-Peptide Fragments,
pubmed-meshheading:2019434-Protein Conformation,
pubmed-meshheading:2019434-Recombinant Proteins,
pubmed-meshheading:2019434-Tetanus Toxin
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pubmed:year |
1991
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pubmed:articleTitle |
Synthesis of tetanus toxin fragment C in insect cells by use of a baculovirus expression system.
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pubmed:affiliation |
Department of Molecular Biology, Wellcome Research Laboratories, Beckenham, Kent, England.
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pubmed:publicationType |
Journal Article
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