Linked Life Data

20188519

Source:http://linkedlifedata.com/resource/pubmed/id/20188519

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2010-3-22
pubmed:databankReference
pubmed:abstractText
The prevalence and characterisation of integrons and the genetic environment of sulphonamide resistance genes were studied in 135 Escherichia coli isolates recovered from blood cultures in a Spanish hospital during 2007. Class 1 and 2 integrons were identified in 54 isolates (intI1, 52 isolates; intI2, 1 isolate; and intI1+intI2, 1 isolate). Of the 53 intI1-positive isolates, 36 (67.9%) contained the classic class 1 integron including the qacEDelta1-sul1 region, and 11 different gene cassette arrangements were demonstrated in 33 of these isolates. Seventeen intI1-positive isolates lacked the qacEDelta1-sul1 region, and 8 gene cassette arrangements were demonstrated in 12 of these isolates. Seventy-one isolates showed a sulphonamide-resistant phenotype, 63 of which contained sul genes. The sul1 gene was associated with intI1 in 36 of 42 sul1-positive isolates, and the sul3 gene was associated with non-classic class 1 integrons in 5 of 7 sul3-positive isolates. Finally, sul2 was found associated with strA-strB genes in 32 of 35 sul2-positive isolates, identifying 11 genetic structures, 1 of them presenting the IS150 element disrupting the strB gene; this structure was included in GenBank with accession no. FJ705354. Almost one-half of the E. coli isolates from blood cultures contained integrons and sul genes. Moreover, sul genes were detected in different structures, one of them new, and could be important determinants in antibiotic resistance dissemination.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1872-7913
pubmed:author
pubmed:copyrightInfo
Copyright 2010 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
492-6
pubmed:meshHeading
pubmed-meshheading:20188519-Anti-Bacterial Agents, pubmed-meshheading:20188519-Bacteremia, pubmed-meshheading:20188519-Bacterial Proteins, pubmed-meshheading:20188519-Blood, pubmed-meshheading:20188519-Carrier Proteins, pubmed-meshheading:20188519-DNA, Bacterial, pubmed-meshheading:20188519-Dihydropteroate Synthase, pubmed-meshheading:20188519-Drug Resistance, Bacterial, pubmed-meshheading:20188519-Escherichia coli, pubmed-meshheading:20188519-Escherichia coli Infections, pubmed-meshheading:20188519-Escherichia coli Proteins, pubmed-meshheading:20188519-Gene Order, pubmed-meshheading:20188519-Genes, Bacterial, pubmed-meshheading:20188519-Hospitals, pubmed-meshheading:20188519-Humans, pubmed-meshheading:20188519-Integrons, pubmed-meshheading:20188519-Microbial Sensitivity Tests, pubmed-meshheading:20188519-Molecular Sequence Data, pubmed-meshheading:20188519-Sequence Analysis, DNA, pubmed-meshheading:20188519-Spain, pubmed-meshheading:20188519-Sulfonamides
pubmed:year
2010
pubmed:articleTitle
Genetic environment of sul genes and characterisation of integrons in Escherichia coli isolates of blood origin in a Spanish hospital.
pubmed:affiliation
Area de Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't