Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2010-6-21
pubmed:abstractText
The AddAB helicase and nuclease complex is used for repairing double-strand DNA breaks in the many bacteria that do not possess RecBCD. Here, we show that AddAB, from the Gram-negative opportunistic pathogen Bacteroides fragilis, can rescue the ultraviolet sensitivity of an Escherichia coli recBCD mutant and that addAB is required for survival of B. fragilis following DNA damage. Using single-molecule observations we demonstrate that AddAB can translocate along DNA at up to 250 bp per second and can unwind an average of 14,000 bp, with some complexes capable of unwinding 40,000 bp. These results demonstrate the importance of processivity for facilitating encounters with recognition sequences that modify enzyme function during homologous recombination.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-10716434, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-11201750, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-11929535, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-12441398, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-12815437, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-12815438, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-1310990, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-15079074, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-15538360, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-15547262, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-15561144, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-15746427, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-15749023, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-16009938, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-16041061, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-16132081, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-16418527, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-16483938, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-16632468, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-17506634, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-17529976, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-17570399, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-17631491, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-18022364, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-18414490, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-18414737, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-18573180, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-18647165, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-19052323, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-19129187, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-19332806, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-19470566, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-19542287, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-19841849, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-19920138, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-2545239, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-2610349, http://linkedlifedata.com/resource/pubmed/commentcorrection/20185564-8068630
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1362-4962
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3721-31
pubmed:dateRevised
2011-8-24
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Single-molecule imaging of Bacteroides fragilis AddAB reveals the highly processive translocation of a single motor helicase.
pubmed:affiliation
EastChem School of Chemistry and COSMIC, The University of Edinburgh, The King's Buildings, Edinburgh EH9 3JR, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't