Linked Life Data

20175145

Source:http://linkedlifedata.com/resource/pubmed/id/20175145

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-2-26
pubmed:abstractText
The toxic effects of ethanol on bovine serum albumin (BSA) were measured by resonance light scattering (RLS), fluorescence spectroscopy, ultraviolet spectrophotometry (UV), circular dichroism (CD), and transmission electron microscopy (TEM). The results indicated that ethanol had toxic effects on BSA, which led to protein denaturation and the effects increased with the ethanol dose. By means of RLS, BSA was found to aggregate in the presence of ethanol and particles smaller than 100 nm were observed from TEM. The fluorescence spectra showed that the intensity of the characteristic peak of BSA decreased and blue shifted, because of changes in the BSA skeleton structure, as well as alteration of the microenvironment of tryptophan (Trp) residues. The conformation changes of BSA were also shown by UV and CD spectrometry.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1099-0461
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
66-71
pubmed:dateRevised
2011-3-18
pubmed:meshHeading
pubmed:articleTitle
Toxic effects of ethanol on bovine serum albumin.
pubmed:affiliation
School of Environmental Science and Engineering, Shandong University, 27 Shanda South Road, Jinan 250100, People's Republic of China. rutaoliu@sdu.edu.cn
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't