Linked Life Data

20174887

Source:http://linkedlifedata.com/resource/pubmed/id/20174887

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2010-8-12
pubmed:abstractText
Ganoderma sinensis fungal immunomodulatory protein (FIP-gsi) was a new member of FIPs family. Based on the cloning of FIP-gsi gene from G. sinensis, this paper reported that FIP-gsi gene was expressed in Escherichia coli expression system. Then, the recombinant proteins were analyzed by the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Finally, the immunomodulatory activity was examined by inducing cytokine genes expression. The results showed that the recombinant FIP-gsi protein could be expressed in E. coli and got the yield of about 25% of the soluble form in the total soluble protein. The FIP-gsi protein was composed of 111 amino acids, and the sequence of homologous rate was 88.6% with FIP-glu (LZ-8). Furthermore, it could enhance the levels of interleukin (IL)-2, IL-3, IL-4, interferon gamma, tumor necrosis factor alpha, and IL-2 receptor (IL-2R) in mouse spleen cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1559-0291
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
162
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1403-13
pubmed:meshHeading
pubmed-meshheading:20174887-Amino Acid Sequence, pubmed-meshheading:20174887-Animals, pubmed-meshheading:20174887-Cytokines, pubmed-meshheading:20174887-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:20174887-Escherichia coli, pubmed-meshheading:20174887-Fungal Proteins, pubmed-meshheading:20174887-Ganoderma, pubmed-meshheading:20174887-Gene Expression Regulation, pubmed-meshheading:20174887-Genetic Vectors, pubmed-meshheading:20174887-Immunologic Factors, pubmed-meshheading:20174887-Mice, pubmed-meshheading:20174887-Molecular Sequence Data, pubmed-meshheading:20174887-Peptides, pubmed-meshheading:20174887-Recombinant Proteins, pubmed-meshheading:20174887-Sequence Alignment, pubmed-meshheading:20174887-Spectrometry, Mass, Matrix-Assisted Laser..., pubmed-meshheading:20174887-Spleen
pubmed:year
2010
pubmed:articleTitle
Cytokines expression induced by Ganoderma sinensis fungal immunomodulatory proteins (FIP-gsi) in mouse spleen cells.
pubmed:affiliation
Plant Biotechnology Research Center, Shanghai Key Laboratory of Agrobiotechnology, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't