20172088

Source:http://linkedlifedata.com/resource/pubmed/id/20172088

Download in:

Switch to

Custom View

Named Graph Language Inference

Statements in which the resource exists as a subject.
Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0079366, umls-concept:C1186983, umls-concept:C1257867, umls-concept:C2936194
pubmed:issue	1
pubmed:dateCreated	2010-2-22
pubmed:abstractText	Microfluidic cell sorter allows efficient separation of small number of cells, which is beneficial in handling cells, especially primary cells that cannot be expanded to large populations. Here, we demonstrate a microfluidic fluorescence-activated cell sorter (muFACS) with a novel sorting mechanism, in which automatic on-chip sorting is realized by turning on/off the hydrodynamic gating valve when a fluorescent target is detected. Formation of the hydrodynamic gating valve was investigated by both numerical simulation and flow visualization experiment. Separation of fluorescent polystyrene beads was then conducted to evaluate this sorting mechanism and to optimize the separation conditions. Isolation of fluorescent HeLa-DsRed cells was further demonstrated with high purity and recovery rate. Viability of the sorted cells was also examined, suggesting a survival rate of more than 90%. We expect this sorting approach to find widespread applications in bioanalysis.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/0370534
pubmed:citationSubset	IM
pubmed:status	MEDLINE
pubmed:month	Mar
pubmed:issn	1873-4324
pubmed:author	pubmed-author:ChenPuP, pubmed-author:FengXiaojunX, pubmed-author:HuntSS, pubmed-author:LiuBi-FengBF, pubmed-author:NiY XYX, pubmed-author:VALJJ
pubmed:copyrightInfo	Copyright 2010 Elsevier B.V. All rights reserved.
pubmed:issnType	Electronic
pubmed:day	17
pubmed:volume	663
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	1-6
pubmed:meshHeading	pubmed-meshheading:20172088-Cell Separation, pubmed-meshheading:20172088-Cell Survival, pubmed-meshheading:20172088-Equipment Design, pubmed-meshheading:20172088-Flow Cytometry, pubmed-meshheading:20172088-HeLa Cells, pubmed-meshheading:20172088-Humans, pubmed-meshheading:20172088-Microfluidics
pubmed:year	2010
pubmed:articleTitle	Hydrodynamic gating valve for microfluidic fluorescence-activated cell sorting.
pubmed:affiliation	Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Systems Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't