Linked Life Data

20170284

Source:http://linkedlifedata.com/resource/pubmed/id/20170284

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-2-22
pubmed:abstractText
The aim of this study was to compare the rate of sperm DNA fragmentation (rSDF; increase of SDF over time) in fresh and gradient isolated frozen-thawed semen samples from male sperm donors of proven fertility. SDF was assessed in the two samples obtained from the same fifteen male donors after 0.5, 1.5, 4.5, 6, 24, 48, and 72 h of incubation in a humidified atmosphere of 5% CO(2) in air at 37 degrees C. Analysis was performed based on chromatin dispersion patterns evaluated using the Halosperm kit. No significant differences in SDF were obtained when fresh and gradient-isolated frozen-thawed spermatozoa were compared at baseline. However, the rSDF shown by the two samples differed and gradient-isolation selected for sperm subpopulations showing a lower variance for SDF. At the individual level, sperm selection by density gradient purification in frozen-thawed samples did not improve the levels of SDF when compared with the values obtained in fresh samples at baseline.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1939-6376
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
27-36
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
DNA fragmentation dynamics in fresh versus frozen thawed plus gradient-isolated human spermatozoa.
pubmed:affiliation
Department of Biology, Universidad Autónoma de Madrid, Madrid, Spain. jaime.gosalvez@uam.es
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't