|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:dateCreated |
2010-3-4
|
|
pubmed:abstractText |
Fibroblast growth factor 21 (FGF21) is a promising drug candidate to combat metabolic diseases. However, high-level expression and purification of recombinant FGF21 (rFGF21) in Escherichia coli (E. coli) is difficult because rFGF21 forms inclusion bodies in the bacteria making it difficult to purify and obtain high concentrations of bioactive rFGF21. To overcome this problem, we fused the FGF21 with SUMO (Small ubiquitin-related modifier) by polymerase chain reaction (PCR), and expressed the fused gene in E. coli BL21(DE3).
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-10858549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-11276432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-11891329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-12057932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-15189146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-15263846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-15902306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-15922331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-15939295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-16084395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-16322573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-16929488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-16936195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-17068132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-17073723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-17550777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-17623664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-18189135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-18467542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-18840786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-19104760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-19208897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-19470704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20163718-19618734
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:issn |
1472-6750
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
10
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
14
|
|
pubmed:dateRevised |
2010-9-30
|
|
pubmed:meshHeading |
pubmed-meshheading:20163718-Animals,
pubmed-meshheading:20163718-Cloning, Molecular,
pubmed-meshheading:20163718-Diabetes Mellitus, Experimental,
pubmed-meshheading:20163718-Escherichia coli,
pubmed-meshheading:20163718-Fibroblast Growth Factors,
pubmed-meshheading:20163718-Genetic Vectors,
pubmed-meshheading:20163718-Humans,
pubmed-meshheading:20163718-Male,
pubmed-meshheading:20163718-Rats,
pubmed-meshheading:20163718-Rats, Wistar,
pubmed-meshheading:20163718-Recombinant Fusion Proteins,
pubmed-meshheading:20163718-Small Ubiquitin-Related Modifier Proteins
|
|
pubmed:year |
2010
|
|
pubmed:articleTitle |
High-level expression and purification of soluble recombinant FGF21 protein by SUMO fusion in Escherichia coli.
|
|
pubmed:affiliation |
Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, China.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
