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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
1991-5-17
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pubmed:abstractText |
We studied the mRNA-binding properties of eukaryotic initiation factor (eIF) 2. This Met-tRNA-binding factor interacts with the cap structure of reoviral mRNA in an ATP-independent manner. Both the beta- and gamma-subunit of eIF-2 are involved in the UV-induced cross-linking of eIF-2 to the cap. The interaction of eIF-2 with a messenger is sensitive to the cap analogue 7-methyl-guanosine 5'-triphosphate as measured by cross-linking and by mRNA retention on nitrocellulose filters. The cap-binding property of eIF-2 does not conflict with the current mRNA-binding model of initiation factors eIF-4A, -4B, and -4F: cross-linking of eIF-4E and of eIF-4B is stimulated by eIF-2. The eIF-2-mediated increase of eIF-4E interaction results in a decrease of the cross-linking of the beta- and gamma-subunits of eIF-2. The presence of GTP in the cross-linking assay interferes with the interaction of eIF-2 with the cap structure but does not inhibit the eIF-2 stimulated eIF-4E and -4B cross-linking. These observations indicate a role for eIF-2 in the mRNA recognition.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Eukaryotic Initiation Factor-2,
http://linkedlifedata.com/resource/pubmed/chemical/Eukaryotic Initiation Factor-4F,
http://linkedlifedata.com/resource/pubmed/chemical/Eukaryotic Initiation Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Initiation Factors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Caps,
http://linkedlifedata.com/resource/pubmed/chemical/eIF-4B
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
266
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7279-84
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2016328-Animals,
pubmed-meshheading:2016328-Cell Line,
pubmed-meshheading:2016328-Eukaryotic Initiation Factor-2,
pubmed-meshheading:2016328-Eukaryotic Initiation Factor-4F,
pubmed-meshheading:2016328-Eukaryotic Initiation Factors,
pubmed-meshheading:2016328-Kinetics,
pubmed-meshheading:2016328-Mammalian orthoreovirus 3,
pubmed-meshheading:2016328-Peptide Initiation Factors,
pubmed-meshheading:2016328-Protein Binding,
pubmed-meshheading:2016328-RNA, Messenger,
pubmed-meshheading:2016328-RNA Caps,
pubmed-meshheading:2016328-Ultraviolet Rays
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pubmed:year |
1991
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pubmed:articleTitle |
Evidence that eukaryotic initiation factor (eIF) 2 is a cap-binding protein that stimulates cap recognition by eIF-4B and eIF-4F.
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pubmed:affiliation |
Department of Molecular Cell Biology, University of Utrecht, The Netherlands.
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pubmed:publicationType |
Journal Article
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