Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
|
pubmed:dateCreated |
1991-5-17
|
pubmed:abstractText |
Binding of neopentylcobalamin and benzylcobalamin to the apoprotein of a haptocorrin from chicken serum has been demonstrated spectrophotometrically. The spectra of the protein-bound cobalamins strongly resemble those of base-on alkylcobalamins and show that when unbound these sterically hindered alkylcobalamins are only approximately 75% (benzyl) and 40% (neopentyl) base-on, at neutral pH and at 5 degrees C. The haptocorrin was found to stabilize the spontaneous thermal decomposition of the neutral species of benzylcobalamin and neopentylcobalamin by 470-fold (3.6 kcal) and 166-fold (3.0 kcal), respectively, relative to the protein-free species. After correction of the activation parameters for the thermal decomposition of the protein-free, neutral alkylcobalamins for the relative proportions of base-on and base-off species, the haptocorrin was found to stabilize the base-on species of both alkylcobalamins by 275- to 1400-fold (approximately 3.3 to 4.3 kcal). From the temperature dependence of the decomposition reactions, the enthalpies of activation are found to be essentially identical for the protein-free and protein-bound species of either cobalamin. Thus, stabilization of the thermal decomposition of these sterically hindered alkylcobalamins by haptocorrin is entirely due to entropic factors.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
0021-9258
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
15
|
pubmed:volume |
266
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
6737-41
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:2016289-Animals,
pubmed-meshheading:2016289-Apoproteins,
pubmed-meshheading:2016289-Calorimetry,
pubmed-meshheading:2016289-Chickens,
pubmed-meshheading:2016289-Drug Stability,
pubmed-meshheading:2016289-Kinetics,
pubmed-meshheading:2016289-Protein Binding,
pubmed-meshheading:2016289-Spectrophotometry,
pubmed-meshheading:2016289-Structure-Activity Relationship,
pubmed-meshheading:2016289-Transcobalamins,
pubmed-meshheading:2016289-Vitamin B 12
|
pubmed:year |
1991
|
pubmed:articleTitle |
Stabilization of thermally labile alkylcobalamins by a haptocorrin from chicken serum.
|
pubmed:affiliation |
Department of Chemistry, Mississippi State University, Mississippi State 39762.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|