Linked Life Data

20162565

Source:http://linkedlifedata.com/resource/pubmed/id/20162565

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-3-1
pubmed:abstractText
The cardiovascular therapeutic potential of bone marrow mesenchymal stromal/stem cells (MSC) is largely mediated by paracrine effects. Traditional preparation of MSC has involved plastic adherence-isolation. In contrast, prospective immunoselection aims to improve cell isolation by enriching for mesenchymal precursor cells (MPC) at higher purity. This study compared the biological characteristics and cardiovascular trophic activity of plastic adherence-isolated MSC (PA-MSC) and MPC prepared from the same human donors by immunoselection for stromal precursor antigen-1 (STRO-1). Compared to PA-MSC, STRO-1-MPC displayed greater (1) clonogenicity, (2) proliferative capacity, (3) multilineage differentiation potential, and (4) mRNA expression of mesenchymal stem cell-related transcripts. In vitro assays demonstrated that conditioned medium from STRO-1-MPC had greater paracrine activity than PA-MSC, with respect to cardiac cell proliferation and migration and endothelial cell migration and tube formation. In keeping with this, STRO-1-MPC exhibited higher gene and protein expression of CXCL12 and HGF. Inhibition of these cytokines attenuated endothelial tube formation and cardiac cell proliferation, respectively. Paracrine responses were enhanced by using supernatant from STRO-1(Bright) MPC and diminished with STRO-1(Dim) conditioned medium. Together, these findings indicate that prospective isolation gives rise to mesenchymal progeny that maintain a higher proportion of immature precursor cells compared to traditional plastic adherence-isolation. Enrichment for STRO-1 is also accompanied by increased expression of cardiovascular-relevant cytokines and enhanced trophic activity. Immunoselection thus provides a strategy for improving the cardiovascular reparative potential of mesenchymal cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1097-4652
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
223
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
530-40
pubmed:meshHeading
pubmed-meshheading:20162565-Antigens, Surface, pubmed-meshheading:20162565-Biological Markers, pubmed-meshheading:20162565-Bone Marrow Cells, pubmed-meshheading:20162565-Cardiovascular Diseases, pubmed-meshheading:20162565-Cell Adhesion, pubmed-meshheading:20162565-Cell Culture Techniques, pubmed-meshheading:20162565-Cell Differentiation, pubmed-meshheading:20162565-Cell Movement, pubmed-meshheading:20162565-Cell Proliferation, pubmed-meshheading:20162565-Cells, Cultured, pubmed-meshheading:20162565-Colony-Forming Units Assay, pubmed-meshheading:20162565-Culture Media, Conditioned, pubmed-meshheading:20162565-Endothelial Cells, pubmed-meshheading:20162565-Humans, pubmed-meshheading:20162565-Immunomagnetic Separation, pubmed-meshheading:20162565-Mesenchymal Stem Cell Transplantation, pubmed-meshheading:20162565-Mesenchymal Stem Cells, pubmed-meshheading:20162565-Multipotent Stem Cells, pubmed-meshheading:20162565-Myocytes, Cardiac, pubmed-meshheading:20162565-Paracrine Communication, pubmed-meshheading:20162565-Plastics, pubmed-meshheading:20162565-RNA, Messenger, pubmed-meshheading:20162565-Regeneration
pubmed:year
2010
pubmed:articleTitle
Enrichment for STRO-1 expression enhances the cardiovascular paracrine activity of human bone marrow-derived mesenchymal cell populations.
pubmed:affiliation
Centre for Stem Cell Research, Robinson Institute and Department of Medicine, University of Adelaide, South Australia, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't