20160675

Source:http://linkedlifedata.com/resource/pubmed/id/20160675

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0035126, umls-concept:C0542341, umls-concept:C0920569, umls-concept:C1569711
pubmed:issue	9
pubmed:dateCreated	2010-5-4
pubmed:abstractText	BACKGROUND.: A neutrophil elastase (NE) inhibitor, Sivelestat, has been approved for the treatment of acute lung injury associated with systemic inflammation in humans. Some reports have also shown its protective effects in liver inflammatory states. We have recently documented the importance of NE in the pathophysiology of liver ischemia/reperfusion injury, a local Ag-independent inflammation response. This study was designed to explore putative cytoprotective functions of clinically available Sivelestat in liver ischemia/reperfusion injury. METHODS.: Partial warm ischemia was produced in the left and middle hepatic lobes of C57BL/6 mice for 90 min, followed by 6 or 24 hr of reperfusion. The mice were given Sivelestat (100 mg/kg, subcutaneous) at 10 min before ischemia, 10 min before reperfusion, and at 1 and 3 hr of reperfusion thereafter. RESULTS.: Sivelestat treatment significantly reduced serum alanine aminotransferase levels and NE activity, when compared with controls. Histological liver examination has revealed that unlike in controls, Sivelestat ameliorated the hepatocellular damage and decreased local neutrophil activity and infiltration. The expression of proinflammatory cytokines (tumor necrosis factor-alpha and interleukin-6), chemokines (CXCL-1, CXCL-2, and CXCL-10), and toll-like receptor 4 was significantly reduced in the treatment group, along with diminished apoptosis through caspase-3 pathway. Moreover, in vitro studies confirmed downregulation of proinflammatory cytokine and chemokine programs in mouse macrophage cell cultures, along with depression of innate toll-like receptor 4 signaling. CONCLUSION.: Sivelestat-mediated NE inhibition may represent an effective therapeutic option in liver transplantation and other inflammation disease states.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/AI23847, http://linkedlifedata.com/resource/pubmed/grant/AI42223, http://linkedlifedata.com/resource/pubmed/grant/R01 DK062357, http://linkedlifedata.com/resource/pubmed/grant/R01 DK062357-08
pubmed:commentsCorrections	http://linkedlifedata.com/resource/pubmed/commentcorrection/20160675-20683430
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/0132144
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL1, http://linkedlifedata.com/resource/pubmed/chemical/Cxcl1 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers, http://linkedlifedata.com/resource/pubmed/chemical/Glycine, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6, http://linkedlifedata.com/resource/pubmed/chemical/Leukocyte Elastase, http://linkedlifedata.com/resource/pubmed/chemical/ONO 5046, http://linkedlifedata.com/resource/pubmed/chemical/Peroxidase, http://linkedlifedata.com/resource/pubmed/chemical/Proteinase Inhibitory Proteins..., http://linkedlifedata.com/resource/pubmed/chemical/RNA, http://linkedlifedata.com/resource/pubmed/chemical/Sulfonamides, http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
pubmed:status	MEDLINE
pubmed:month	May
pubmed:issn	1534-6080
pubmed:author	pubmed-author:BusuttilRonald WRW, pubmed-author:FreitasMaria Cecilia SMC, pubmed-author:Kupiec-WeglinskiJerzy WJW, pubmed-author:UchidaYoichiroY, pubmed-author:ZhaoDanyunD
pubmed:issnType	Electronic
pubmed:day	15
pubmed:volume	89
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	1050-6
pubmed:dateRevised	2011-1-14
pubmed:meshHeading	pubmed-meshheading:20160675-Animals, pubmed-meshheading:20160675-Chemokine CXCL1, pubmed-meshheading:20160675-DNA Primers, pubmed-meshheading:20160675-Glycine, pubmed-meshheading:20160675-Humans, pubmed-meshheading:20160675-Immunohistochemistry, pubmed-meshheading:20160675-Inflammation, pubmed-meshheading:20160675-Interleukin-6, pubmed-meshheading:20160675-Leukocyte Elastase, pubmed-meshheading:20160675-Liver, pubmed-meshheading:20160675-Male, pubmed-meshheading:20160675-Mice, pubmed-meshheading:20160675-Mice, Inbred C57BL, pubmed-meshheading:20160675-Neutrophils, pubmed-meshheading:20160675-Peroxidase, pubmed-meshheading:20160675-Polymerase Chain Reaction, pubmed-meshheading:20160675-Proteinase Inhibitory Proteins, Secretory, pubmed-meshheading:20160675-RNA, pubmed-meshheading:20160675-Reperfusion Injury, pubmed-meshheading:20160675-Sulfonamides, pubmed-meshheading:20160675-Tumor Necrosis Factor-alpha
pubmed:year	2010
pubmed:articleTitle	The protective function of neutrophil elastase inhibitor in liver ischemia/reperfusion injury.
pubmed:affiliation	Dumont-UCLA Transplant Center, Division of Liver and Pancreas Transplantation, Department of Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural