|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1991-5-23
|
|
pubmed:abstractText |
Engagement of the clonotypic antigen receptor (TCR) on T lymphocytes provokes an activation response leading to cell proliferation and lymphokine secretion. To examine the molecular basis of T cell signaling, we generated transgenic animals in which a lymphocyte-specific nonreceptor protein-tyrosine kinase p59fyn(T) is 20-fold overexpressed in developing T lineage cells. Thymocytes from these mice, analyzed using both cellular and biochemical assays, were remarkably hyperstimulable. Moreover, the responsiveness of normal thymocytes to TCR-derived signals correlated well with the extent to which p59fyn was expressed in these cells. Overexpression of a catalytically inactive form of p59fyn substantially inhibited TCR-mediated activation in otherwise normal thymocytes. These effects are unique to p59fyn; overexpression of a closely related T cell-specific tyrosine kinase, p56lck, elicits dramatically different phenotypes. Our results suggest that p59fyn is a critically important component of the TCR signal transduction apparatus.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/FYN protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fyn protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Protein pp60(v-src),
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fyn,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0092-8674
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
19
|
|
pubmed:volume |
65
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
281-91
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:2015626-Animals,
pubmed-meshheading:2015626-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:2015626-Calcium,
pubmed-meshheading:2015626-Cells, Cultured,
pubmed-meshheading:2015626-Genetic Vectors,
pubmed-meshheading:2015626-Humans,
pubmed-meshheading:2015626-Lymphocyte Activation,
pubmed-meshheading:2015626-Mice,
pubmed-meshheading:2015626-Mice, Inbred C57BL,
pubmed-meshheading:2015626-Mice, Inbred DBA,
pubmed-meshheading:2015626-Mice, Transgenic,
pubmed-meshheading:2015626-Oncogene Protein pp60(v-src),
pubmed-meshheading:2015626-Phenotype,
pubmed-meshheading:2015626-Protein-Tyrosine Kinases,
pubmed-meshheading:2015626-Proto-Oncogene Proteins,
pubmed-meshheading:2015626-Proto-Oncogene Proteins c-fyn,
pubmed-meshheading:2015626-RNA, Messenger,
pubmed-meshheading:2015626-Receptors, Antigen, T-Cell,
pubmed-meshheading:2015626-Signal Transduction,
pubmed-meshheading:2015626-T-Lymphocyte Subsets,
pubmed-meshheading:2015626-T-Lymphocytes
|
|
pubmed:year |
1991
|
|
pubmed:articleTitle |
Regulation of T cell receptor signaling by a src family protein-tyrosine kinase (p59fyn).
|
|
pubmed:affiliation |
Howard Hughes Medical Institute, University of Washington, Seattle 98195.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
