Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1991-5-23
pubmed:abstractText
1. 5-Hydroxytryptamine (5-HT) produced a concentration-dependent increase in the membrane concentration of 1,2-diacylglycerol (DG) in the rabbit isolated basilar artery, but did not stimulate the hydrolysis of membrane phosphoinositide. 2. The 5-HT-induced accumulation of DG could be blocked with the putative phospholipase C inhibitor 2-nitro-4-carboxyphenyl-N,N-diphenylcarbamate (NCDC; 70 microM), but not with the protein kinase C inhibitor, 1-(5-isoquinolinesulphonyl)-2-methyl piperazine (H7; 50 microM). 3. Direct stimulation of protein kinase C with phorbol 12,13-dibutyrate (PDBu) produced sustained smooth muscle contraction which was fairly rapid in onset and could be reversed by H7 but not by NCDC. The inactive phorbol, 4 alpha phorbol 12,13-dideceonate, did not produce contraction in the basilar artery. 4. 5-HT-induced contractions (1 nM-100 microM) were blocked or greatly reduced in the presence of the protein kinase inhibitor H7 or polymyxin B, and with the phospholipase C inhibitor, NCDC. The concentrations of these inhibitors which abolished contraction to 5-HT, did not alter smooth muscle contraction produced in response to 30 mM K(+)-physiological salt solution (PSS). 5. These data suggest that DG production and the subsequent activation of PKC forms an important component of the cerebrovascular contractile response to 5-HT. As the DG does not appear to arise from membrane phosphatidylinositol, it appears that 5-HT can stimulate the production of this second messenger in cerebral arteries by a mechanism which is different from peripheral arteries.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-2154130, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-2410594, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-2414429, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-2439892, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-2443210, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-2556108, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-2560978, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-2674488, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-2726426, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-2737287, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-2737723, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-2758222, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-2812040, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-2857490, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-2875415, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-2963816, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-2991913, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-2992666, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-2997209, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-3007748, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-3016238, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-3045562, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-3304132, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-3313065, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-3346836, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-3446783, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-3462727, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-3463215, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-3463304, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-3468750, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-3558493, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-37533, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-3818651, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-3932351, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-3955301, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-6146314, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-6147898, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-6205237, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-6238627, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-6301427, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-6431975, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-7214673, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-7270700, http://linkedlifedata.com/resource/pubmed/commentcorrection/2015423-7391002
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0007-1188
pubmed:author
pubmed:issnType
Print
pubmed:volume
102
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
415-21
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
5-hydroxytryptamine-stimulated accumulation of 1,2-diacylglycerol in the rabbit basilar artery: a role for protein kinase C in smooth muscle contraction.
pubmed:affiliation
Department of Physiology and Pharmacology, University of Southampton.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't