Linked Life Data

20154140

Source:http://linkedlifedata.com/resource/pubmed/id/20154140

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2010-3-26
pubmed:abstractText
Eukaryotic mRNA translation begins with recruitment of the 40S ribosome complex to the mRNA 5' end through the eIF4F initiation complex binding to the 5' m(7)G-mRNA cap. Spliced leader (SL) RNA trans splicing adds a trimethylguanosine (TMG) cap and a sequence, the SL, to the 5' end of mRNAs. Efficient translation of TMG-capped mRNAs in nematodes requires the SL sequence. Here we define a core set of nucleotides and a stem-loop within the 22-nucleotide nematode SL that stimulate translation of mRNAs with a TMG cap. The structure and core nucleotides are conserved in other nematode SLs and correspond to regions of SL1 required for early Caenorhabditis elegans development. These SL elements do not facilitate translation of m(7)G-capped RNAs in nematodes or TMG-capped mRNAs in mammalian or plant translation systems. Similar stem-loop structures in phylogenetically diverse SLs are predicted. We show that the nematode eukaryotic translation initiation factor 4E/G (eIF4E/G) complex enables efficient translation of the TMG-SL RNAs in diverse in vitro translation systems. TMG-capped mRNA translation is determined by eIF4E/G interaction with the cap and the SL RNA, although the SL does not increase the affinity of eIF4E/G for capped RNA. These results suggest that the mRNA 5' untranslated region (UTR) can play a positive and novel role in translation initiation through interaction with the eIF4E/G complex in nematodes and raise the issue of whether eIF4E/G-RNA interactions play a role in the translation of other eukaryotic mRNAs.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1098-5549
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1958-70
pubmed:dateRevised
2010-10-4
pubmed:meshHeading
pubmed-meshheading:20154140-5' Untranslated Regions, pubmed-meshheading:20154140-Animals, pubmed-meshheading:20154140-Base Sequence, pubmed-meshheading:20154140-Caenorhabditis elegans, pubmed-meshheading:20154140-Caenorhabditis elegans Proteins, pubmed-meshheading:20154140-Cell-Free System, pubmed-meshheading:20154140-Eukaryotic Initiation Factor-4E, pubmed-meshheading:20154140-Eukaryotic Initiation Factor-4G, pubmed-meshheading:20154140-Guanosine, pubmed-meshheading:20154140-Molecular Sequence Data, pubmed-meshheading:20154140-Nucleic Acid Conformation, pubmed-meshheading:20154140-Protein Biosynthesis, pubmed-meshheading:20154140-RNA, Messenger, pubmed-meshheading:20154140-RNA Cap Analogs, pubmed-meshheading:20154140-RNA Stability, pubmed-meshheading:20154140-Sequence Alignment, pubmed-meshheading:20154140-Trans-Splicing
pubmed:year
2010
pubmed:articleTitle
The nematode eukaryotic translation initiation factor 4E/G complex works with a trans-spliced leader stem-loop to enable efficient translation of trimethylguanosine-capped RNAs.
pubmed:affiliation
Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO 80045, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural