Linked Life Data

20148434

Source:http://linkedlifedata.com/resource/pubmed/id/20148434

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2010-2-22
pubmed:abstractText
We followed the development of the nitric oxide-cyclic guanosine monophosphate (NO-cGMP) system during locust embryogenesis in whole mount nervous systems and brain sections by using various cytochemical techniques. We visualized NO-sensitive neurons by cGMP immunofluorescence after incubation with an NO donor in the presence of the soluble guanylyl cyclase (sGC) activator YC-1 and the phosphodiesterase-inhibitor isobutyl-methyl-xanthine (IBMX). Central nervous system (CNS) cells respond to NO as early as 38% embryogenesis. By using the NADPH-diaphorase technique, we identified somata and neurites of possible NO-synthesizing cells in the CNS. The first NADPH-diaphorase-positive cell bodies appear around 40% embryogenesis in the brain and at 47% in the ventral nerve cord. The number of positive cells reaches the full complement of adult cells at 80%. In the brain, some structures, e.g., the mushroom bodies acquire NADPH-diaphorase staining only postembryonically. Immunolocalization of L-citrulline confirmed the presence of NOS in NADPH-diaphorase-stained neurons and, in addition, indicated enzymatic activity in vivo. In whole mount ventral nerve cords, citrulline immunolabeling was present in varying subsets of NADPH-diaphorase-positive cells, but staining was very variable and often weak. However, in a regeneration paradigm in which one of the two connectives between ganglia had been crushed, strong, reliable staining was observed as early as 60% embryogenesis. Thus, citrulline immunolabeling appears to reflect specific activity of NOS. However, in younger embryos, NOS may not always be constitutively active or may be so at a very low level, below the citrulline antibody detection threshold. For the CNS, histochemical markers for NOS do not provide conclusive evidence for a developmental role of this enzyme.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1096-9861
pubmed:author
pubmed:copyrightInfo
(c) 2009 Wiley-Liss, Inc.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
518
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1157-75
pubmed:meshHeading
pubmed-meshheading:20148434-1-Methyl-3-isobutylxanthine, pubmed-meshheading:20148434-Animals, pubmed-meshheading:20148434-Brain, pubmed-meshheading:20148434-Citrulline, pubmed-meshheading:20148434-Cyclic GMP, pubmed-meshheading:20148434-Embryo, Nonmammalian, pubmed-meshheading:20148434-Enzyme Activators, pubmed-meshheading:20148434-Ganglia, Invertebrate, pubmed-meshheading:20148434-Indazoles, pubmed-meshheading:20148434-Locusta migratoria, pubmed-meshheading:20148434-NADPH Dehydrogenase, pubmed-meshheading:20148434-Nerve Regeneration, pubmed-meshheading:20148434-Nervous System, pubmed-meshheading:20148434-Neurites, pubmed-meshheading:20148434-Neurons, pubmed-meshheading:20148434-Neuropil, pubmed-meshheading:20148434-Nitric Oxide, pubmed-meshheading:20148434-Nitric Oxide Synthase, pubmed-meshheading:20148434-Phosphodiesterase Inhibitors, pubmed-meshheading:20148434-Signal Transduction
pubmed:year
2010
pubmed:articleTitle
Development of nitrergic neurons in the nervous system of the locust embryo.
pubmed:affiliation
Cell Biology, Institute of Physiology, University of Veterinary Medicine Hannover, D-30173 Hannover, Germany. Michael.stern@tiho-hannover.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't