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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1991-5-15
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pubmed:databankReference |
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pubmed:abstractText |
We have cloned the gene for the chicken erythroid transcription factor GATA-1 (formerly Eryf1, NF-E1, or GF-1). The gene is composed of six exons, two of which encode the two finger domains of the protein. Transcription of GATA-1 in chicken embryonic erythroid cells initiates from multiple sites clustered approximately 200 base pairs upstream from the start of protein-coding sequence. A number of sequence motifs for known DNA-binding proteins are found to be protected in DNase I-footprinting experiments by either erythroid or brain nuclear extracts or by both. Notably, a cluster of three GATA-1 sites is protected by the erythroid extract, as well as by purified GATA-1. We find that the upstream region of the gene functions as a powerful promoter when transfected into embryonic erythroid cells. In primary chicken embryo fibroblasts the promoter exhibits lower activity, which is increased when the cells are cotransfected with a second plasmid expressing the GATA-1 cDNA. The results suggest that GATA-1 protein plays an autoregulatory role in its own expression.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-1979040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-1990287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-2104960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-2142999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-2154480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-2249770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-2276623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-2300555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-2320112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-2402439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-2461328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-2467208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-2546677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-2577875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-2620826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-2644044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-2725678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-2739739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-2776214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-2887293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-3166139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-3208750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-3209071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-3309569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-3396073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-3413070,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-6279674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2014222-6315951
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3004-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2014222-Animals,
pubmed-meshheading:2014222-Base Sequence,
pubmed-meshheading:2014222-Chickens,
pubmed-meshheading:2014222-Cloning, Molecular,
pubmed-meshheading:2014222-DNA-Binding Proteins,
pubmed-meshheading:2014222-Erythrocytes,
pubmed-meshheading:2014222-Exons,
pubmed-meshheading:2014222-Gene Expression Regulation,
pubmed-meshheading:2014222-Genes,
pubmed-meshheading:2014222-Molecular Sequence Data,
pubmed-meshheading:2014222-Promoter Regions, Genetic,
pubmed-meshheading:2014222-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:2014222-Transcription Factors,
pubmed-meshheading:2014222-Zinc Fingers
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pubmed:year |
1991
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pubmed:articleTitle |
Structure and promoter activity of the gene for the erythroid transcription factor GATA-1.
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pubmed:affiliation |
Biomolecular Sciences Division, King's College, London, England.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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