Source:http://linkedlifedata.com/resource/pubmed/id/20139775
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2010-2-24
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| pubmed:abstractText |
Myeloid-leukemic cells (AML, MDS, CML) can be differentiated to leukemia-derived dendritic cell [DC (DCleu)] potentially presenting the whole leukemic antigen repertoire without knowledge of distinct leukemia antigens and are regarded as promising candidates for a vaccination strategy. We studied the capability of 6 serum-free DC culture methods, chosen according to different mechanisms, to induce DC differentiation in 137 cases of AML and 52 cases of MDS. DC-stimulating substances were cytokines ("standard-medium", "MCM-Mimic", "cytokine-method"), bacterial lysates ("Picibanil"), double-stranded RNA ["Poly (I:C)"] or a cytokine bypass method ("Ca-ionophore"). The quality/quantity of DC generated was estimated by flow cytometry studying (co) expressions of "DC"antigens, costimulatory, maturation, and blast-antigens. Comparing these methods on average 15% to 32% DC, depending on methods used, could be obtained from blast-containing mononuclear cells (MNC) in AML/MDS cases with a DC viability of more than 60%. In all, 39% to 64% of these DC were mature; 31% to 52% of leukemic blasts could be converted to DCleu and DCleu-proportions in the suspension were 2% to 70% (13%). Average results of all culture methods tested were comparable, however not every given case of AML could be differentiated to DC with 1 selected method. However performing a pre-analysis with 3 DC-generating methods (MCM-Mimic, Picibanil, Ca-ionophore) we could generate DC in any given case. Functional analyses provided proof, that DC primed T cells to antileukemia-directed cytotoxic cells, although an anti-leukemic reaction was not achieved in every case. In summary our data show that a successful, quantitative DC/DCleu generation is possible with the best of 3 previously tested methods in any given case. Reasons for different functional behaviors of DC-primed T cells must be evaluated to design a practicable DC-based vaccination strategy.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Cancer Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Picibanil,
http://linkedlifedata.com/resource/pubmed/chemical/Poly I-C
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| pubmed:status |
MEDLINE
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| pubmed:issn |
1537-4513
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| pubmed:author |
pubmed-author:DressigJuliaJ,
pubmed-author:GrabruckerChristineC,
pubmed-author:KolbHans JochemHJ,
pubmed-author:KremserAndreasA,
pubmed-author:KroellTanjaT,
pubmed-author:KufnerStefanieS,
pubmed-author:LiepertAnjaA,
pubmed-author:SalihHelmutH,
pubmed-author:SchmetzerHelgaH,
pubmed-author:SchmidChristophC,
pubmed-author:SchollNinaN,
pubmed-author:TischerJohannaJ
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| pubmed:issnType |
Electronic
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| pubmed:volume |
33
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
185-99
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| pubmed:meshHeading |
pubmed-meshheading:20139775-Adult,
pubmed-meshheading:20139775-Aged,
pubmed-meshheading:20139775-Aged, 80 and over,
pubmed-meshheading:20139775-Antigen Presentation,
pubmed-meshheading:20139775-Antigens, Differentiation,
pubmed-meshheading:20139775-Antigens, Neoplasm,
pubmed-meshheading:20139775-Cancer Vaccines,
pubmed-meshheading:20139775-Cell Culture Techniques,
pubmed-meshheading:20139775-Cell Differentiation,
pubmed-meshheading:20139775-Cell Separation,
pubmed-meshheading:20139775-Culture Media, Serum-Free,
pubmed-meshheading:20139775-Cytokines,
pubmed-meshheading:20139775-Cytotoxicity, Immunologic,
pubmed-meshheading:20139775-Dendritic Cells,
pubmed-meshheading:20139775-Female,
pubmed-meshheading:20139775-Flow Cytometry,
pubmed-meshheading:20139775-Humans,
pubmed-meshheading:20139775-Leukemia, Myeloid, Acute,
pubmed-meshheading:20139775-Male,
pubmed-meshheading:20139775-Middle Aged,
pubmed-meshheading:20139775-Myelodysplastic Syndromes,
pubmed-meshheading:20139775-Picibanil,
pubmed-meshheading:20139775-Poly I-C,
pubmed-meshheading:20139775-T-Lymphocytes, Cytotoxic
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| pubmed:articleTitle |
Dendritic cells (DCs) can be successfully generated from leukemic blasts in individual patients with AML or MDS: an evaluation of different methods.
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| pubmed:affiliation |
Medical Department III, University Hospital Grosshadern, Ludwig-Maximilians-University, 81377 Munich, Germany.
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| pubmed:publicationType |
Journal Article
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