Linked Life Data

20136694

Source:http://linkedlifedata.com/resource/pubmed/id/20136694

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-5-7
pubmed:abstractText
RFamide related peptides (RFRPs) have been extensively implicated in the neuroendocrine control of reproduction. While steroid hormones strongly regulate the closely-related kisspeptin gene and protein expression, the regulation of RFRPs or their receptor by steroid hormones is almost unknown. The present study aimed to quantify relative levels of RFRP and Kiss1 gene expression and their G protein-coupled receptors (GPR147 and GPR54, respectively) in various brain areas and the pituitary gland, and to determine the effects of differing levels of oestradiol and pubertal development on levels of these gene products. In Experiment 1, the treatment groups examined were: dioestrus, ovariectomised and ovariectomised with replacement oestradiol to induce a preovulatory-like luteinising hormone surge. Micropunched brain regions and whole pituitary glands were processed for measurement of RFRP, Kiss1, GPR147 and GPR54 mRNA by quantitative reverse transcriptase-polymerase chain reaction. As expected, Kiss1 gene expression was low in the rostral periventricular area of the third ventricle of ovariectomised animals, whereas levels were highest in the arcuate nucleus in this situation. No such oestrogenic effects were observed for RFRP gene expression. GPR147 gene expression was highest in the rostral periventricular region of the third ventricle. The levels of GPR147 and GPR54 mRNA were markedly lower in the pituitary gland than in the hypothalamic regions, and RFRP and Kiss1 mRNA were virtually undetectable in the pituitary gland. These data imply that the actions of RFamides are likely to be predominantly central in nature. In Experiment 2, hypothalamic RFRP and GPR147 mRNA levels were measured in male and female rats aged 2, 4, 6 and 8 weeks. In females, RFRP gene expression increased with developmental age, peaking around the time of puberty, whereas in males gene expression increased between 2 and 4 weeks of age. These results suggest a role in the regulation of adult reproduction rather that prepubertal infertility.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1365-2826
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
309-16
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:20136694-Animals, pubmed-meshheading:20136694-Brain, pubmed-meshheading:20136694-Estradiol, pubmed-meshheading:20136694-Female, pubmed-meshheading:20136694-Gene Expression Profiling, pubmed-meshheading:20136694-Gene Expression Regulation, Developmental, pubmed-meshheading:20136694-Kisspeptins, pubmed-meshheading:20136694-Luteinizing Hormone, pubmed-meshheading:20136694-Male, pubmed-meshheading:20136694-Models, Biological, pubmed-meshheading:20136694-Neuropeptides, pubmed-meshheading:20136694-Ovariectomy, pubmed-meshheading:20136694-Pituitary Gland, pubmed-meshheading:20136694-Proteins, pubmed-meshheading:20136694-Rats, pubmed-meshheading:20136694-Rats, Sprague-Dawley, pubmed-meshheading:20136694-Receptors, G-Protein-Coupled, pubmed-meshheading:20136694-Receptors, Neuropeptide, pubmed-meshheading:20136694-Sexual Maturation, pubmed-meshheading:20136694-Steroids
pubmed:year
2010
pubmed:articleTitle
Developmental and steroidogenic effects on the gene expression of RFamide related peptides and their receptor in the rat brain and pituitary gland.
pubmed:affiliation
Centre for Neuroendocrinology and Departments of Anatomy and Structural Biology, University of Otago School of Medical Sciences, Dunedin, New Zealand.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't