Source:http://linkedlifedata.com/resource/pubmed/id/20129744
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3-4
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| pubmed:dateCreated |
2010-3-29
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| pubmed:abstractText |
Immunization using recombinant maltose binding protein-gonadotropin releasing hormone (MBP-GnRH6) altered both testicular development and transcription of the pituitary GnRH receptor (GnRHR) gene in boars. Scrotal measurement and blood samples were taken at 4-week interval after immunization at 9 weeks of age. The concentrations of testosterone and anti-GnRH antibodies in serum were determined by radioimmunoassay and enzyme-linked immunosorbent assay, respectively. The results showed that active immunization with MBP-GnRH6 increased the serum concentration of anti-GnRH antibodies (P<0.05) and reduced the serum concentration of testosterone (P<0.05) as compared with MBP controls. At 25 weeks of age, boars were sacrificed and testes were evaluated histologically. Testicular development was suppressed in the MBP-GnRH6 immunized animals as compared with MBP immunized boars. MBP-GnRH6 immunized pigs exhibited mounting behavior 4 weeks later than MBP immunized boars. No mature spermatozoa were observed from MBP-GnRH6 immunized animals. By real-time quantitative PCR analysis, the amount of GnRHR mRNA in the pituitary tissue was found to be significantly lower in MBP-GnRH6 immunized animals than in controls (P<0.05). These data demonstrate that recombinant MBP-GnRH6 was effective in immunological castration in boars.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Gonadotropin-Releasing Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Maltose-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Periplasmic Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, LHRH,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Testosterone
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
1873-2232
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright (c) 2010 Elsevier B.V. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
119
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
275-81
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:20129744-Animals,
pubmed-meshheading:20129744-Antibodies,
pubmed-meshheading:20129744-Gonadotropin-Releasing Hormone,
pubmed-meshheading:20129744-Male,
pubmed-meshheading:20129744-Maltose-Binding Proteins,
pubmed-meshheading:20129744-Periplasmic Binding Proteins,
pubmed-meshheading:20129744-Pituitary Gland,
pubmed-meshheading:20129744-Polymerase Chain Reaction,
pubmed-meshheading:20129744-RNA, Messenger,
pubmed-meshheading:20129744-Receptors, LHRH,
pubmed-meshheading:20129744-Recombinant Fusion Proteins,
pubmed-meshheading:20129744-Sexual Behavior, Animal,
pubmed-meshheading:20129744-Spermatozoa,
pubmed-meshheading:20129744-Sterilization, Reproductive,
pubmed-meshheading:20129744-Swine,
pubmed-meshheading:20129744-Testis,
pubmed-meshheading:20129744-Testosterone,
pubmed-meshheading:20129744-Vaccination
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| pubmed:year |
2010
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| pubmed:articleTitle |
Active immunization with recombinant GnRH fusion protein in boars reduces both testicular development and mRNA expression levels of GnRH receptor in pituitary.
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| pubmed:affiliation |
Department of Animal Medicine, College of Animal Science and Technology, Anhui Agricultural University, No. 130 Changjiang West Road, Hefei, Anhui, PR China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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