pubmed-article:20124352 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:20124352 | lifeskim:mentions | umls-concept:C0013846 | lld:lifeskim |
pubmed-article:20124352 | lifeskim:mentions | umls-concept:C0577559 | lld:lifeskim |
pubmed-article:20124352 | lifeskim:mentions | umls-concept:C1514468 | lld:lifeskim |
pubmed-article:20124352 | lifeskim:mentions | umls-concept:C0872252 | lld:lifeskim |
pubmed-article:20124352 | lifeskim:mentions | umls-concept:C0183396 | lld:lifeskim |
pubmed-article:20124352 | lifeskim:mentions | umls-concept:C1709634 | lld:lifeskim |
pubmed-article:20124352 | lifeskim:mentions | umls-concept:C0443131 | lld:lifeskim |
pubmed-article:20124352 | pubmed:issue | 5 | lld:pubmed |
pubmed-article:20124352 | pubmed:dateCreated | 2010-5-4 | lld:pubmed |
pubmed-article:20124352 | pubmed:abstractText | We demonstrate a new approach for internal mass calibration on an electron transfer dissociation-enabled linear ion trap-orbitrap hybrid mass spectrometer. Fluoranthene cations, a byproduct of the reaction used for generation of electron transfer dissociation reagent anions, are co-injected with the analyte cations in all orbitrap mass analysis events. The fluoranthene cations serve as a robust internal calibrant with minimal impact on scan time (<20 ms) or spectral quality. Following external mass calibration, 60 replicate LC-MS/MS runs of a complex peptide mixture were collected over the course of approximately 136 h (almost 6 days). Using only standard external mass calibration, the mass accuracy for a typical analysis was -3.31 +/- 0.93 ppm (sigma) for precursors and -2.32 +/- 0.89 ppm for products. After application of internal recalibration, mass accuracy improved to +0.77 +/- 0.71 ppm for precursors and +0.17 +/- 0.67 ppm for products. When all 60 replicate runs were analyzed together without internal mass recalibration, the mass accuracy was -1.23 +/- 1.54 ppm for precursors and -0.18 +/- 1.42 ppm for products, nearly a 2-fold drop in precision relative to an individual run. After internal mass recalibration, this improved to +0.80 +/- 0.70 ppm for precursors and +0.16 +/- 0.67 ppm for products, roughly equivalent to that obtained in a single run, demonstrating a near complete elimination of mass calibration drift. | lld:pubmed |
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pubmed-article:20124352 | pubmed:language | eng | lld:pubmed |
pubmed-article:20124352 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:20124352 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:20124352 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:20124352 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:20124352 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:20124352 | pubmed:month | May | lld:pubmed |
pubmed-article:20124352 | pubmed:issn | 1535-9484 | lld:pubmed |
pubmed-article:20124352 | pubmed:author | pubmed-author:CoonJoshua... | lld:pubmed |
pubmed-article:20124352 | pubmed:author | pubmed-author:XiaQiangweiQ | lld:pubmed |
pubmed-article:20124352 | pubmed:author | pubmed-author:McAlisterGrae... | lld:pubmed |
pubmed-article:20124352 | pubmed:author | pubmed-author:WengerCraig... | lld:pubmed |
pubmed-article:20124352 | pubmed:issnType | Electronic | lld:pubmed |
pubmed-article:20124352 | pubmed:volume | 9 | lld:pubmed |
pubmed-article:20124352 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:20124352 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:20124352 | pubmed:pagination | 754-63 | lld:pubmed |
pubmed-article:20124352 | pubmed:dateRevised | 2011-7-28 | lld:pubmed |
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pubmed-article:20124352 | pubmed:meshHeading | pubmed-meshheading:20124352... | lld:pubmed |
pubmed-article:20124352 | pubmed:year | 2010 | lld:pubmed |
pubmed-article:20124352 | pubmed:articleTitle | Sub-part-per-million precursor and product mass accuracy for high-throughput proteomics on an electron transfer dissociation-enabled orbitrap mass spectrometer. | lld:pubmed |
pubmed-article:20124352 | pubmed:affiliation | Department Chemistry, University of Wisconsin, Madison, Wisconsin 53706, USA. | lld:pubmed |
pubmed-article:20124352 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:20124352 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |