Linked Life Data

20121079

Source:http://linkedlifedata.com/resource/pubmed/id/20121079

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2010-2-19
pubmed:abstractText
In force spectroscopy single-molecule experiments, an individual molecule, usually a polymer, is mechanically stretched by means of an externally controlled driving potential. Typically, the stiffness of this potential is much smaller than the stiffness of the potential of mean force along the molecular extension coordinate. Here we discuss how such a disparity alters the free energy and the reversibility of the driven system, with respect to the pristine molecular system under examination. In particular, by simulating unfolding/refolding experiments of a small protein, we examine the traits of the potential of mean force that are responsible for the dramatic amount of work dissipated in experiments using a soft device. Finally, we show that in bidirectional experiments the free energy of the free molecular system can be easily recovered by appropriate reweighting methods.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1520-5207
pubmed:author
pubmed:issnType
Electronic
pubmed:day
25
pubmed:volume
114
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2509-16
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Free energy reconstruction in bidirectional force spectroscopy experiments: the effect of the device stiffness.
pubmed:affiliation
Dipartimento di Chimica, Universita di Firenze, Via della Lastruccia 3, I-50019 Sesto Fiorentino, Italy.
pubmed:publicationType
Journal Article