rdf:type |
|
lifeskim:mentions |
umls-concept:C0007589,
umls-concept:C0008300,
umls-concept:C0033414,
umls-concept:C0034790,
umls-concept:C0037083,
umls-concept:C0039194,
umls-concept:C0079189,
umls-concept:C0085358,
umls-concept:C1332717,
umls-concept:C1413244,
umls-concept:C1511938,
umls-concept:C1521902,
umls-concept:C1706438,
umls-concept:C1710082,
umls-concept:C1881379,
umls-concept:C2698600
|
pubmed:issue |
3
|
pubmed:dateCreated |
2010-2-16
|
pubmed:abstractText |
Immature CD4(+)CD8(+) (double-positive (DP)) thymocytes are signaled via T cell antigen receptors (TCRs) to undergo positive selection and become responsive to intrathymic cytokines such as interleukin 7 (IL-7). We report here that cytokine signaling is required for positively selected thymocytes to express the transcription factor Runx3, specify CD8 lineage choice and differentiate into cytotoxic-lineage T cells. In DP thymocytes genetically engineered to be cytokine responsive, IL-7 signaling induced TCR-unsignaled DP thymocytes to express Runx3 and to differentiate into mature CD8(+) T cells, completely circumventing positive selection. We conclude that TCR-mediated positive selection converts DP cells into cytokine-responsive thymocytes, but it is subsequent signaling by intrathymic cytokines that specifies CD8 lineage choice and promotes differentiation into cytotoxic-lineage T cells.
|
pubmed:commentsCorrections |
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
|
pubmed:issn |
1529-2916
|
pubmed:author |
pubmed-author:AdoroStanleyS,
pubmed-author:AlagAmala SAS,
pubmed-author:CatalfamoMartaM,
pubmed-author:CuiYongzhiY,
pubmed-author:ErmanBatuB,
pubmed-author:FeigenbaumLionelL,
pubmed-author:GressRonald ERE,
pubmed-author:GuinterTerryT,
pubmed-author:HennighausenLotharL,
pubmed-author:KimuraMotoko YMY,
pubmed-author:KuboMasatoM,
pubmed-author:LucasPhilip JPJ,
pubmed-author:ParkJung-HyunJH,
pubmed-author:SingerAlfredA
|
pubmed:issnType |
Electronic
|
pubmed:volume |
11
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
257-64
|
pubmed:meshHeading |
pubmed-meshheading:20118929-Animals,
pubmed-meshheading:20118929-Cell Count,
pubmed-meshheading:20118929-Cell Differentiation,
pubmed-meshheading:20118929-Cell Lineage,
pubmed-meshheading:20118929-Core Binding Factor Alpha 3 Subunit,
pubmed-meshheading:20118929-Cytokines,
pubmed-meshheading:20118929-Flow Cytometry,
pubmed-meshheading:20118929-Interleukin-7,
pubmed-meshheading:20118929-Mice,
pubmed-meshheading:20118929-Mice, Knockout,
pubmed-meshheading:20118929-Mice, Transgenic,
pubmed-meshheading:20118929-STAT5 Transcription Factor,
pubmed-meshheading:20118929-Signal Transduction,
pubmed-meshheading:20118929-T-Lymphocytes, Cytotoxic
|
pubmed:year |
2010
|
pubmed:articleTitle |
Signaling by intrathymic cytokines, not T cell antigen receptors, specifies CD8 lineage choice and promotes the differentiation of cytotoxic-lineage T cells.
|
pubmed:affiliation |
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
|
pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Intramural
|