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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2010-2-8
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pubmed:abstractText |
Hepatitis C virus (HCV), which infects 2-3% of the world population, is a causative agent of chronic hepatitis and the leading indication for liver transplantation. The ability to propagate HCV in cell culture (HCVcc) is a relatively recent breakthrough and a key tool in the quest for specific antiviral therapeutics. Monitoring HCV infection in culture generally involves bulk population assays, use of genetically modified viruses and/or terminal processing of potentially precious samples. Here we develop a cell-based fluorescent reporter system that allows sensitive distinction of individual HCV-infected cells in live or fixed samples. We demonstrate use of this technology for several previously intractable applications, including live-cell imaging of viral propagation and host response, as well as visualizing infection of primary hepatocyte cultures. Integration of this reporter with modern image-based analysis methods could open new doors for HCV research.
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pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/,
http://linkedlifedata.com/resource/pubmed/grant/1 R01 DK085713-01,
http://linkedlifedata.com/resource/pubmed/grant/DK081193,
http://linkedlifedata.com/resource/pubmed/grant/F32 DK081193-01A1,
http://linkedlifedata.com/resource/pubmed/grant/F32 DK081193-02,
http://linkedlifedata.com/resource/pubmed/grant/F32 DK081193-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI075099,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI075099-01A1,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI075099-02,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI075099-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK056966-01,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK056966-02,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK056966-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK056966-04,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK056966-05A2,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK056966-06,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK056966-07,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK056966-09,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK085713-01,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK085713-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK56966
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-10544172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-10786833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-12356718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-12554735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-12642610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-15483230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-15710892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-15919912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-15947137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-16122679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-16125763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-16127453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-16153868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-16177806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-16301520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-16520386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-16585524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-16641454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-16651538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-17287280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-17325668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-17941058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-17959627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-18005747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-18026090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-19003912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-19088272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-19182773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-20432262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20118917-9371625
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1546-1696
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pubmed:author |
pubmed-author:BhatiaSangeeta NSN,
pubmed-author:CataneseMaria TeresaMT,
pubmed-author:JonesChristopher TCT,
pubmed-author:KhetaniSalman RSR,
pubmed-author:LawLok Man JLM,
pubmed-author:MacDonaldMargaret RMR,
pubmed-author:OhThomas STS,
pubmed-author:PlossAlexanderA,
pubmed-author:RiceCharles MCM,
pubmed-author:SchogginsJohn WJW,
pubmed-author:SyderAndrew JAJ
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pubmed:issnType |
Electronic
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pubmed:volume |
28
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
167-71
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pubmed:dateRevised |
2011-8-1
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pubmed:meshHeading |
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pubmed:year |
2010
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pubmed:articleTitle |
Real-time imaging of hepatitis C virus infection using a fluorescent cell-based reporter system.
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pubmed:affiliation |
Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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