20117076

pubmed:Citation

5

In this work we have investigated the effect of a pathogenic mitochondrial DNA mutation found in human colon cells, at a functional-molecular level. The mutation results in the amino-acid substitution Tyr19His in subunit I of the human CytcO and it is associated with respiratory deficiency. It was introduced into Rhodobacter sphaeroides, which carries a cytochrome c oxidase (cytochrome aa(3)) that serves as a model of the mitochondrial counterpart. The residue is situated in the middle of a pathway that is used to transfer substrate protons as well as protons that are pumped across the membrane. The Tyr33His (equivalent residue in the bacterial CytcO) structural variant of the enzyme was purified and its function was investigated. The results show that in the structurally altered CytcO the activity decreased due to slowed proton transfer; proton transfer from an internal proton donor, the highly-conserved Glu286, to the catalytic site was slowed by a factor of approximately 5, while reprotonation of the Glu from solution was slowed by a factor of approximately 40. In addition, in the structural variant proton pumping was completely impaired. These results are explained in terms of introduction of a barrier for proton transfer through the D pathway and changes in the coordination of water molecules surrounding the Glu286 residue. The study offers an explanation, at the molecular level, to the link between a specific amino-acid substitution and a pathogenic phenotype identified in human colon cells.

eng

IM

MEDLINE

0006-3002

Print

NLM

550-6

pubmed-meshheading:20117076-Amino Acid Substitution, pubmed-meshheading:20117076-Catalysis, pubmed-meshheading:20117076-Catalytic Domain, pubmed-meshheading:20117076-Colonic Neoplasms, pubmed-meshheading:20117076-DNA, Mitochondrial, pubmed-meshheading:20117076-Electron Transport Complex IV, pubmed-meshheading:20117076-Humans, pubmed-meshheading:20117076-Kinetics, pubmed-meshheading:20117076-Mitochondria, pubmed-meshheading:20117076-Models, Molecular, pubmed-meshheading:20117076-Mutagenesis, Site-Directed, pubmed-meshheading:20117076-Mutation, pubmed-meshheading:20117076-Oxygen, pubmed-meshheading:20117076-Protein Conformation, pubmed-meshheading:20117076-Proton Pumps, pubmed-meshheading:20117076-Protons, pubmed-meshheading:20117076-Rhodobacter sphaeroides, pubmed-meshheading:20117076-Structure-Activity Relationship

A pathogenic mutation in cytochrome c oxidase results in impaired proton pumping while retaining O(2)-reduction activity.

Journal Article, Research Support, Non-U.S. Gov't