Linked Life Data

20116659

Source:http://linkedlifedata.com/resource/pubmed/id/20116659

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-2-1
pubmed:abstractText
Dietary components, like beta-glucans, can modulate the intestinal immune response. We previously showed that fecal water enriched with oat beta-glucan stimulated the cytokine-induced immune response of enterocytes. It is, however, unclear whether beta-glucans activate nuclear factor-kappaB (NF-kappaB) pathways in the intestine in vivo and if so, whether enterocytes, intestinal leukocytes, or both respond to beta-glucans. We evaluated the effects of an oral gavage of 3 mg dietary oat (1-->3), (1-->4)-beta-D-glucans that was administered twice daily during 3.5 days on intestinal NF-kappaB transactivation and subsequent cytokine production of intestinal leukocytes and enterocytes in 16 NF-kappaB reporter mice. We hypothesized that oat beta-glucan activates the central immune transcription factor NF-kappaB and increased cytokine secretion, as we previously reported immune stimulating effects by oat beta-glucan. We found that mice that were administered oat beta-glucans (n = 8) showed an increased intestinal NF-kappaB transactivation in leukocytes (P = .021) and enterocytes (P = .012), particularly in the proximal part of the small intestine (ileum), as compared to placebo mice (n = 8). Surprisingly, NF-kappaB was not activated in the colon. Furthermore, the level of interleukin 12 was increased in intestinal lysates from all compartments, whereas the concentration of interferon gamma was decreased in the proximal small intestine (P = .046). Finally, tumor necrosis factor alpha showed a trend toward a reduced production in the colon (P = .057). Because we have earlier shown that human enterocyte cell lines do not express the beta-glucan receptor dectin-1 in vitro, we now conclude that after consumption, dietary oat beta-glucans most likely firstly activate the intestinal leukocytes, which in turn increases cellular activation of enterocytes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1879-0739
pubmed:author
pubmed:copyrightInfo
2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
40-8
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Dietary (1-->3), (1-->4)-beta-D-glucans from oat activate nuclear factor-kappaB in intestinal leukocytes and enterocytes from mice.
pubmed:affiliation
Department of Human Biology, Maastricht University Medical Centre+, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't