Linked Life Data

2011395

Source:http://linkedlifedata.com/resource/pubmed/id/2011395

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1991-5-3
pubmed:abstractText
Simian virus 40 large T antigen exposed at the cell surface is oligomerized and phosphorylated in a mature fashion. NP40-soluble surface T antigen, prepared from infected cells, sediments mainly as a tetramer and has a nuclear phosphorylation pattern. Since these are modifications that occur over several hours, surface T appears to be a population of older molecules. This is in apparent contradiction to kinetic studies showing that T antigen arrives at the surface in 20 min. We present a model to account for this apparent discrepancy. It postulates that both newly-synthesized and older, mature molecules are transported to the surface. The newly-synthesized molecules are highly unstable and lost rapidly from the surface whereas the mature molecules are stable and accumulate over time. This stability may be due to oligomerization and/or phosphorylation or to an interaction with other surface molecules that act as anchors. Our model further proposes that most of the T antigen in transit to the surface is newly-synthesized protein from the cytoplasm, while the majority of the T antigen at the surface is mature protein from the nucleus.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
379-87
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Evidence that SV40 surface tumor antigen originates from the nucleus and cytoplasm.
pubmed:affiliation
School of Life and Health Sciences, University of Delaware, Newark 19716.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.