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rdf:type |
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lifeskim:mentions |
umls-concept:C0007589,
umls-concept:C0017262,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0029418,
umls-concept:C0086982,
umls-concept:C0279266,
umls-concept:C0870134,
umls-concept:C1314939,
umls-concept:C1511938,
umls-concept:C1513143,
umls-concept:C1515877,
umls-concept:C1879547,
umls-concept:C2349975
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pubmed:issue |
6
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pubmed:dateCreated |
2010-4-7
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pubmed:abstractText |
Here, we show the involvement of signaling pathways to induce the gene expression of bone morphogenetic protein (BMP) in the osteogenic activity of physcion-8-O-beta-D-glucopyranoside (physcion-Glu); it stimulated osteoblast differentiation in mouse osteoblast MC3T3-E1 subclone 4 cells and induced BMP-2 gene expression and activation of Akt and ERK/MAP kinases. Physcion-Glu-induced BMP-2 expression and mineralization were attenuated by LY294002, an inhibitor of PI3K that lies upstream of Akt and MAP kinases, suggesting that physcion-Glu induces osteoblast differentiation via PI3K-Akt/MAP kinase signaling pathways, which play important roles in inducing BMP-2 gene expression. Physcion-Glu also enhanced BMP-2-induced commitment of mouse bi-potential mesenchymal precursor C2C12 cells into osteoblasts while inducing the transcription of several osteogenic BMP isoforms, such as BMP-2, -4, -7, and -9. Osteogenic synergy between BMP-2 and physcion-Glu was supported by the fact that noggin inhibited BMP-2 and physcion-Glu-induced alkaline phosphatase expression and activity. Considering that physcion-Glu induced Runx2 activity and the nuclear translocation of p-Smad, physcion-Glu could act by enhancing the BMP signaling pathway that induces Smad activation and translocation to activate Runx2. In conclusion, physcion-Glu could enhance the commitment of mesenchymal progenitors into osteoblasts and their differentiation by activating signaling pathways to induce BMP gene expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1097-4644
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pubmed:author |
pubmed-author:ChoiJe-YongJY,
pubmed-author:ChoiYeon HeeYH,
pubmed-author:KimHyun-NamHN,
pubmed-author:KimSeong HwanSH,
pubmed-author:KimYoung SupYS,
pubmed-author:KwakHan BokHB,
pubmed-author:LeeSu-UiSU,
pubmed-author:LimKyung-EunKE,
pubmed-author:MinYong KiYK,
pubmed-author:ParkSang-JoonSJ,
pubmed-author:RheeMyungchullM
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
109
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1148-57
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:20108254-Animals,
pubmed-meshheading:20108254-Blotting, Western,
pubmed-meshheading:20108254-Bone Morphogenetic Protein 2,
pubmed-meshheading:20108254-Cell Differentiation,
pubmed-meshheading:20108254-Cell Line,
pubmed-meshheading:20108254-Chromones,
pubmed-meshheading:20108254-Emodin,
pubmed-meshheading:20108254-Glucosides,
pubmed-meshheading:20108254-Mesenchymal Stem Cells,
pubmed-meshheading:20108254-Mice,
pubmed-meshheading:20108254-Morpholines,
pubmed-meshheading:20108254-Osteoblasts,
pubmed-meshheading:20108254-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:20108254-Polymerase Chain Reaction,
pubmed-meshheading:20108254-Promoter Regions, Genetic,
pubmed-meshheading:20108254-Signal Transduction
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pubmed:year |
2010
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pubmed:articleTitle |
Physcion-8-O-beta-D-glucopyranoside enhances the commitment of mouse mesenchymal progenitors into osteoblasts and their differentiation: Possible involvement of signaling pathways to activate BMP gene expression.
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pubmed:affiliation |
Laboratory of Chemical Genomics, Korea Research Institute of Chemical Technology, Daejeon 305-600, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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