Linked Life Data

20103130

Source:http://linkedlifedata.com/resource/pubmed/id/20103130

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2010-1-27
pubmed:abstractText
A simple, rapid, and sensitive determination of total free thiol groups in biological samples using cerium (IV) as a fluorescence probe is reported. The protocol is based on the oxidation of thiols by Ce(IV) and the formation the Ce(III) disulfide complex, which gives a fluorescence enhancement of Ce(III) at 352 nm. Using glutathione (GSH) and cysteine as model compounds, incubation with Ce(IV) at 25 degrees C for 6 min results in fluorescence, whose intensity is proportional to the thiol concentration in the range of 1.00-160 nM. The detection limits for GSH and cysteine are 0.05 and 0.08 nM, respectively. Other common metal ions and amino acids have little interference to the thiol detection. Cu(II) was used as a fluorescence quencher to eliminate potential interference from tryptophan. The method has been successfully applied to assays of free thiol contents in pig liver tissue samples, with a RSD lower than 2.5% and recovery between 100.6% and 102.3%.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1873-4324
pubmed:author
pubmed:copyrightInfo
Copyright 2009 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
5
pubmed:volume
659
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
238-42
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Spectrofluorimetric determination of total free thiols based on formation of complexes of Ce(III) with disulfide bonds.
pubmed:affiliation
College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't