Linked Life Data

20102553

Source:http://linkedlifedata.com/resource/pubmed/id/20102553

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2010-6-22
pubmed:abstractText
Endothelial progenitor cells (EPCs) may contribute to rejection and cardiac allograft vasculopathy (CAV) by being intrinsically involved in the rejection process and causing neointimal hyperplasia. The mammalian target of rapamycin inhibitors (mTORi), sirolimus and everolimus, have been demonstrated to attenuate the progression of CAV and are cytotoxic to EPC. Thus, one mechanism by which mTORi may protect against CAV is by altering EPC function. Our study measured circulating EPC function and correlated this assessment with rejection episodes in heart transplant (HT) recipients. In addition, we examined the effect of mTORi on EPCs. Patients who received HT at our institution between 1995 and 2007 were included and stratified by International Society for Heart and Lung Transplantation (ISHLT) rejection grade. Group A (n = 13) consisted of patients with at least one moderate/severe rejection episode (grade > or = 2). Group B (n = 28) patients had no moderate/severe episodes (grade < 2). Patients were also independently stratified based on exposure as mTORi (n = 21) vs. non mTORi (n = 20). To assess EPC functional capacity, we counted the number of colony-forming units (CFU) of EPCs in peripheral blood samples from HT recipients. There were no significant differences in baseline characteristics between groups. The mean EPC-CFU counts/plate for group A (rejecting) were 30 +/- 6 vs.16 +/- 3 for group B (nonrejecting) (P = 0.03). The EPC-CFU counts/plate in the mTORi group (15 +/- 3) were lower compared to the non mTORi (27 +/- 5) group (P = 0.04). We found that EPC colony-forming capacity was higher in HT patients who experienced moderate/severe rejection episodes. Patients on mTORi showed a reduced EPC colony count consistent with our previous findings of EPC cytotoxicity. Detection of circulating EPC function post-transplant may reliably identify patient risk level for subsequent allograft rejection and allow for appropriate adjustments to immunosuppression. Converting to mTORi therapy may reduce EPC function and provide a novel mechanism to prevent rejection and possibly attenuate the development of CAV.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1432-2277
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
641-8
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:20102553-Adult, pubmed-meshheading:20102553-Aged, pubmed-meshheading:20102553-Colony-Forming Units Assay, pubmed-meshheading:20102553-Endothelial Cells, pubmed-meshheading:20102553-Female, pubmed-meshheading:20102553-Graft Rejection, pubmed-meshheading:20102553-Heart Transplantation, pubmed-meshheading:20102553-Humans, pubmed-meshheading:20102553-Immunosuppressive Agents, pubmed-meshheading:20102553-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:20102553-Male, pubmed-meshheading:20102553-Middle Aged, pubmed-meshheading:20102553-Protein-Serine-Threonine Kinases, pubmed-meshheading:20102553-Retrospective Studies, pubmed-meshheading:20102553-Risk, pubmed-meshheading:20102553-Sirolimus, pubmed-meshheading:20102553-Stem Cells, pubmed-meshheading:20102553-TOR Serine-Threonine Kinases
pubmed:year
2010
pubmed:articleTitle
Correlation between circulating endothelial progenitor cell function and allograft rejection in heart transplant patients.
pubmed:affiliation
Heart Transplant Program, Peter Munk Cardiac Centre, Toronto General Hospital, University Health Network, Toronto, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't