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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2010-2-18
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pubmed:abstractText |
CCL3 is a protein of the CC chemokine family known to be important for T cell recruitment in inflammatory diseases. The aim of the current study was to evaluate the effects and putative mechanism of action of evasin-1, a novel CCL3-binding protein, in the pathogenesis of acute graft-versus-host disease (GVHD). GVHD was induced by the transplantation of splenocytes from C57BL/6J to B6D2F1 mice. Treatment of recipient mice with evasin-1 prevented mortality associated with GVHD. This was correlated with reduced weight loss and clinical disease severity. Analysis of the small intestine showed that evasin-1 treatment reduced the histopathological score and decreased levels of IFN-gamma and CCL5. Mechanistically, evasin-1 treatment reduced the number of CD4(+) and CD8(+) T cells infiltrating the small intestine, as assessed by immunohistochemistry, and the adhesion of leukocytes to intestinal venules of recipient mice, as assessed by intravital microscopy. Evasin-1 was also able to decrease liver damage, as seen by reduction of inflammatory infiltrate and IFN-gamma levels. Treatment with evasin-1 did not interfere with graft-versus-leukemia. Altogether, our studies demonstrate that CCL3 plays a major role in mediating GVHD, but not graft-versus-leukemia in mice and suggest that blockade of CCL3 with evasin-1 has potential therapeutic application in patients undergoing bone marrow transplantation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Inflammatory Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Ccl3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Ccl5 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL3,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL5,
http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone,
http://linkedlifedata.com/resource/pubmed/chemical/Evasin-1, Rhipicephalus sanguineus,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Inflammatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Chemokine
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1550-6606
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pubmed:author |
pubmed-author:AlessandriAna LAL,
pubmed-author:ArantesRosa M ERM,
pubmed-author:CastorMarina G MMG,
pubmed-author:FagundesCaio TCT,
pubmed-author:PinhoVanessaV,
pubmed-author:ProudfootAmanda E IAE,
pubmed-author:ResendeCarolina BCB,
pubmed-author:RezendeBárbaraB,
pubmed-author:SilvaTarcília ATA,
pubmed-author:SousaLirlândia PLP,
pubmed-author:SouzaDanielle GDG,
pubmed-author:TeixeiraMauro MMM
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
184
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2646-54
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pubmed:meshHeading |
pubmed-meshheading:20100934-Animals,
pubmed-meshheading:20100934-Anti-Inflammatory Agents,
pubmed-meshheading:20100934-CD4-Positive T-Lymphocytes,
pubmed-meshheading:20100934-CD8-Positive T-Lymphocytes,
pubmed-meshheading:20100934-Cell Transplantation,
pubmed-meshheading:20100934-Chemokine CCL3,
pubmed-meshheading:20100934-Chemokine CCL5,
pubmed-meshheading:20100934-Dexamethasone,
pubmed-meshheading:20100934-Female,
pubmed-meshheading:20100934-Graft vs Host Disease,
pubmed-meshheading:20100934-Graft vs Leukemia Effect,
pubmed-meshheading:20100934-Immunohistochemistry,
pubmed-meshheading:20100934-Interferon-gamma,
pubmed-meshheading:20100934-Intestine, Small,
pubmed-meshheading:20100934-Macrophage Inflammatory Proteins,
pubmed-meshheading:20100934-Male,
pubmed-meshheading:20100934-Mice,
pubmed-meshheading:20100934-Mice, Inbred C57BL,
pubmed-meshheading:20100934-Mice, Inbred DBA,
pubmed-meshheading:20100934-Mice, Inbred Strains,
pubmed-meshheading:20100934-Mice, Transgenic,
pubmed-meshheading:20100934-Receptors, Chemokine,
pubmed-meshheading:20100934-Spleen
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pubmed:year |
2010
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pubmed:articleTitle |
The CCL3/macrophage inflammatory protein-1alpha-binding protein evasin-1 protects from graft-versus-host disease but does not modify graft-versus-leukemia in mice.
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pubmed:affiliation |
Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, BeloHorizonte, Brazil.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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