| pubmed-article:20099325 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20099325 | lifeskim:mentions | umls-concept:C0376432 | lld:lifeskim |
| pubmed-article:20099325 | lifeskim:mentions | umls-concept:C1261322 | lld:lifeskim |
| pubmed-article:20099325 | lifeskim:mentions | umls-concept:C1523924 | lld:lifeskim |
| pubmed-article:20099325 | lifeskim:mentions | umls-concept:C0037812 | lld:lifeskim |
| pubmed-article:20099325 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:20099325 | pubmed:dateCreated | 2010-1-29 | lld:pubmed |
| pubmed-article:20099325 | pubmed:abstractText | The purpose of this study was to combine a three-dimensional NMR-compatible bioreactor with hyperpolarized (13)C NMR spectroscopy in order to probe cellular metabolism in real time. JM1 (immortalized rat hepatoma) cells were cultured in a three-dimensional NMR-compatible fluidized bioreactor. (31)P spectra were acquired before and after each injection of hyperpolarized [1-(13)C] pyruvate and subsequent (13)C spectroscopy at 11.7 T. (1)H and two-dimensional (1)H-(1)H-total correlation spectroscopy spectra were acquired from extracts of cells grown in uniformly labeled (13)C-glucose, on a 16.4 T, to determine (13)C fractional enrichment and distribution of (13)C label. JM1 cells were found to have a high rate of aerobic glycolysis in both two-dimensional culture and in the bioreactor, with 85% of the (13)C label from uniformly labeled (13)C-glucose being present as either lactate or alanine after 23 h. Flux measurements of pyruvate through lactate dehydrogenase and alanine aminotransferase in the bioreactor system were 12.18 +/- 0.49 nmols/sec/10(8) cells and 2.39 +/- 0.30 nmols/sec/10(8) cells, respectively, were reproducible in the same bioreactor, and were not significantly different over the course of 2 days. Although this preliminary study involved immortalized cells, this combination of technologies can be extended to the real-time metabolic exploration of primary benign and cancerous cells and tissues prior to and after therapy. | lld:pubmed |
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| pubmed-article:20099325 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20099325 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20099325 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:20099325 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20099325 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:20099325 | pubmed:issn | 1522-2594 | lld:pubmed |
| pubmed-article:20099325 | pubmed:author | pubmed-author:MacdonaldJeff... | lld:pubmed |
| pubmed-article:20099325 | pubmed:author | pubmed-author:WilsonDavid... | lld:pubmed |
| pubmed-article:20099325 | pubmed:author | pubmed-author:KurhanewiczJo... | lld:pubmed |
| pubmed-article:20099325 | pubmed:author | pubmed-author:VigneronDanie... | lld:pubmed |
| pubmed-article:20099325 | pubmed:author | pubmed-author:DewarBrian... | lld:pubmed |
| pubmed-article:20099325 | pubmed:author | pubmed-author:KeshariKayvan... | lld:pubmed |
| pubmed-article:20099325 | pubmed:author | pubmed-author:Van... | lld:pubmed |
| pubmed-article:20099325 | pubmed:author | pubmed-author:JeffriesRex... | lld:pubmed |
| pubmed-article:20099325 | pubmed:author | pubmed-author:ZierhutMatthe... | lld:pubmed |
| pubmed-article:20099325 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20099325 | pubmed:volume | 63 | lld:pubmed |
| pubmed-article:20099325 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20099325 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20099325 | pubmed:pagination | 322-9 | lld:pubmed |
| pubmed-article:20099325 | pubmed:dateRevised | 2011-1-7 | lld:pubmed |
| pubmed-article:20099325 | pubmed:meshHeading | pubmed-meshheading:20099325... | lld:pubmed |
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| pubmed-article:20099325 | pubmed:meshHeading | pubmed-meshheading:20099325... | lld:pubmed |
| pubmed-article:20099325 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20099325 | pubmed:articleTitle | Hyperpolarized (13)C spectroscopy and an NMR-compatible bioreactor system for the investigation of real-time cellular metabolism. | lld:pubmed |
| pubmed-article:20099325 | pubmed:affiliation | Department of Radiology, University of California San Francisco, SanFrancisco, California, USA. | lld:pubmed |
| pubmed-article:20099325 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20099325 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:20099325 | lld:pubmed |
