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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2010-1-29
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pubmed:abstractText |
The purpose of this study was to combine a three-dimensional NMR-compatible bioreactor with hyperpolarized (13)C NMR spectroscopy in order to probe cellular metabolism in real time. JM1 (immortalized rat hepatoma) cells were cultured in a three-dimensional NMR-compatible fluidized bioreactor. (31)P spectra were acquired before and after each injection of hyperpolarized [1-(13)C] pyruvate and subsequent (13)C spectroscopy at 11.7 T. (1)H and two-dimensional (1)H-(1)H-total correlation spectroscopy spectra were acquired from extracts of cells grown in uniformly labeled (13)C-glucose, on a 16.4 T, to determine (13)C fractional enrichment and distribution of (13)C label. JM1 cells were found to have a high rate of aerobic glycolysis in both two-dimensional culture and in the bioreactor, with 85% of the (13)C label from uniformly labeled (13)C-glucose being present as either lactate or alanine after 23 h. Flux measurements of pyruvate through lactate dehydrogenase and alanine aminotransferase in the bioreactor system were 12.18 +/- 0.49 nmols/sec/10(8) cells and 2.39 +/- 0.30 nmols/sec/10(8) cells, respectively, were reproducible in the same bioreactor, and were not significantly different over the course of 2 days. Although this preliminary study involved immortalized cells, this combination of technologies can be extended to the real-time metabolic exploration of primary benign and cancerous cells and tissues prior to and after therapy.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20099325-11494221,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20099325-9608589
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1522-2594
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
322-9
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pubmed:dateRevised |
2011-1-7
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pubmed:meshHeading |
pubmed-meshheading:20099325-Animals,
pubmed-meshheading:20099325-Bioreactors,
pubmed-meshheading:20099325-Carbon Isotopes,
pubmed-meshheading:20099325-Carcinoma, Hepatocellular,
pubmed-meshheading:20099325-Cell Culture Techniques,
pubmed-meshheading:20099325-Cell Line, Tumor,
pubmed-meshheading:20099325-Equipment Design,
pubmed-meshheading:20099325-Equipment Failure Analysis,
pubmed-meshheading:20099325-Magnetic Resonance Spectroscopy,
pubmed-meshheading:20099325-Neoplasm Proteins,
pubmed-meshheading:20099325-Rats,
pubmed-meshheading:20099325-Reproducibility of Results,
pubmed-meshheading:20099325-Sensitivity and Specificity
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pubmed:year |
2010
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pubmed:articleTitle |
Hyperpolarized (13)C spectroscopy and an NMR-compatible bioreactor system for the investigation of real-time cellular metabolism.
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pubmed:affiliation |
Department of Radiology, University of California San Francisco, SanFrancisco, California, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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