|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
7
|
|
pubmed:dateCreated |
2010-2-17
|
|
pubmed:abstractText |
A novel method is proposed for direct detection of DNA hybridization on microarrays. Optical interferometry is used for label-free sensing of biomolecular accumulation on glass surfaces, enabling dynamic detection of interactions. Capabilities of the presented method are demonstrated by high-throughput sensing of solid-phase hybridization of oligonucleotides. Hybridization of surface immobilized probes with 20 base pair-long target oligonucleotides was detected by comparing the label-free microarray images taken before and after hybridization. Through dynamic data acquisition during denaturation by washing the sample with low ionic concentration buffer, melting of duplexes with a single-nucleotide mismatch was distinguished from perfectly matching duplexes with high confidence interval (>97%). The presented technique is simple, robust, and accurate, and eliminates the need of using labels or secondary reagents to monitor the oligonucleotide hybridization.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-10084107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-10090733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-10521349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-10572185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-10931914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-10976070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-11737946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-11812850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-11823860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-12624187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-12904946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-14987092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-15265725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-15301950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-15319319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-15829238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-15913384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-15986697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-16262683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-16380191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-16680197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-17369420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-17947320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-18040987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-18523019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-18707136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-19061409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-19628383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-7569999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-9592175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20097056-9843981
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
1873-4235
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
(c) 2010 Elsevier B.V. All rights reserved.
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
15
|
|
pubmed:volume |
25
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1789-95
|
|
pubmed:dateRevised |
2011-7-26
|
|
pubmed:meshHeading |
pubmed-meshheading:20097056-Base Pair Mismatch,
pubmed-meshheading:20097056-DNA Mutational Analysis,
pubmed-meshheading:20097056-Equipment Design,
pubmed-meshheading:20097056-Equipment Failure Analysis,
pubmed-meshheading:20097056-In Situ Hybridization,
pubmed-meshheading:20097056-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:20097056-Point Mutation,
pubmed-meshheading:20097056-Polymorphism, Single Nucleotide,
pubmed-meshheading:20097056-Reproducibility of Results,
pubmed-meshheading:20097056-Sensitivity and Specificity,
pubmed-meshheading:20097056-Staining and Labeling
|
|
pubmed:year |
2010
|
|
pubmed:articleTitle |
Label-free microarray imaging for direct detection of DNA hybridization and single-nucleotide mismatches.
|
|
pubmed:affiliation |
Department of Electrical & Computer Engineering, Boston University, Boston, MA 02215, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Evaluation Studies,
Research Support, N.I.H., Extramural
|
