Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1978-1-27
pubmed:abstractText
The production of collagenase (EC 3.4.24.3) by endotoxin-stimulated macrophages was significantly inhibited by indomethacin, indicating that prostaglandins (PGs) mediate this effect. Inhibitions of collagenase production by indomethacin was overcome by addition of exogenous PGE2 at 10 nM whereas the addition of 0.1 and 1.0 micrometer PGE2 increased the enzyme production to 3 times that achieved by endotoxin. Although the addition of prostaglandin alone to macrophage cultures did not stimulate collagenase production, the simultaneous addition of PGE1 or PGE2 and endotoxin enhanced collagenase activity 2- to 10-fold. This increase was detectable at PGE concentrations of 10 nM and was optimal at 0.1-1.0 micrometer. PGF2alpha had little effect on either the enhancement of collagenase production by endotoxin-stimulated macrophages or its restoration in cultures inhibited by indomethacin. Radioimmunoassay of prostaglandins in the culture media revealed that macrophages exposed to endotoxin secreted 40-fold more PGE2 than did unstimulated cells. The increase in PGE2 was detected 4 hr after exposure to endotoxin and was maximal at 14 hr. The peak PGE2 concentrations in the culture media were similar to those of exogenous PGE2 (about 10 nM) needed to restore collagenase production in indomethacin-treated cultures. These findings demonstrate the involvement of PGE in the endotoxin-activation of macrophages with resultant production of collagenase.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/200941-1184744, http://linkedlifedata.com/resource/pubmed/commentcorrection/200941-163038, http://linkedlifedata.com/resource/pubmed/commentcorrection/200941-163864, http://linkedlifedata.com/resource/pubmed/commentcorrection/200941-169722, http://linkedlifedata.com/resource/pubmed/commentcorrection/200941-176663, http://linkedlifedata.com/resource/pubmed/commentcorrection/200941-240303, http://linkedlifedata.com/resource/pubmed/commentcorrection/200941-4287120, http://linkedlifedata.com/resource/pubmed/commentcorrection/200941-4322078, http://linkedlifedata.com/resource/pubmed/commentcorrection/200941-4351450, http://linkedlifedata.com/resource/pubmed/commentcorrection/200941-4368514, http://linkedlifedata.com/resource/pubmed/commentcorrection/200941-4368515, http://linkedlifedata.com/resource/pubmed/commentcorrection/200941-4368516, http://linkedlifedata.com/resource/pubmed/commentcorrection/200941-4371998, http://linkedlifedata.com/resource/pubmed/commentcorrection/200941-4372628, http://linkedlifedata.com/resource/pubmed/commentcorrection/200941-4455511, http://linkedlifedata.com/resource/pubmed/commentcorrection/200941-4458866, http://linkedlifedata.com/resource/pubmed/commentcorrection/200941-4595580, http://linkedlifedata.com/resource/pubmed/commentcorrection/200941-5063598, http://linkedlifedata.com/resource/pubmed/commentcorrection/200941-5924404, http://linkedlifedata.com/resource/pubmed/commentcorrection/200941-958392
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4955-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
Prostaglandin regulation of macrophage collagenase production.
pubmed:publicationType
Journal Article