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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2010-5-5
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pubmed:abstractText |
Herein, we describe the optimization of a linked enzyme assay suitable for high-throughput screening of decarboxylases, a target family whose activity has historically been difficult to quantify. Our approach uses a commercially available bicarbonate detection reagent to measure decarboxylase activity. The assay is performed in a fully enclosed automated screening system under inert nitrogen atmosphere to minimize perturbation by exogenous CO2. Receiver operating characteristic (ROC) analysis following a pilot screen of a small library of approximately 3,600 unique molecules for inhibitors of Trypanosoma brucei ornithine decarboxylase quantitatively demonstrates that the assay has excellent discriminatory power (area under the curve = 0.90 with 95% confidence interval between 0.82 and 0.97).
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-10051176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-10069996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-11683631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-11911991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-12429091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-12653650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-15476392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-17464296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-17504214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-1765093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-19413567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-2037868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-2106270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-3036823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-3128541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-3924048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-3938090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-6422008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-6818954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-7490260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-7947774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-8704306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20085486-9109665
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1557-8127
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
175-85
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pubmed:dateRevised |
2011-7-28
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pubmed:meshHeading |
pubmed-meshheading:20085486-Animals,
pubmed-meshheading:20085486-Bicarbonates,
pubmed-meshheading:20085486-Carboxy-Lyases,
pubmed-meshheading:20085486-Data Interpretation, Statistical,
pubmed-meshheading:20085486-Drug Evaluation, Preclinical,
pubmed-meshheading:20085486-Enzyme Assays,
pubmed-meshheading:20085486-Enzyme Inhibitors,
pubmed-meshheading:20085486-Malate Dehydrogenase,
pubmed-meshheading:20085486-Ornithine Decarboxylase,
pubmed-meshheading:20085486-Phosphoenolpyruvate Carboxylase,
pubmed-meshheading:20085486-ROC Curve,
pubmed-meshheading:20085486-Trypanosoma brucei brucei
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pubmed:year |
2010
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pubmed:articleTitle |
Optimization of a non-radioactive high-throughput assay for decarboxylase enzymes.
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pubmed:affiliation |
Graduate Program in Chemistry and Chemical Biology, University of California, San Francisco, California, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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