Linked Life Data

20082579

Source:http://linkedlifedata.com/resource/pubmed/id/20082579

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2011-1-6
pubmed:abstractText
Potent immunoadjuvants are needed to elicit responses following mucosal delivery. PLGA (poly[D,L-lactic-co-glycolic acid]) nanospheres, Quillaja saponin (QS) and cross-linked dextran microspheres (CDM) as drug delivery and absorption enhancer adjuvants were evaluated. PLGA nanospheres were prepared by solvent evaporation method. Particulate characteristics of nanospheres were studied by optical and scanning electron microscopes and dynamic light scattering technique. The mean diameter of nanospheres encapsulated with TT and TT?+?QS determined as 425 and 390?nm. Loadings of TT and QS were 30?±?1.9% and 23?±?2.8%. Nanospheres encapsulated with TT or QS were intranasally administered to rabbits, three times in two-week intervals and the serum IgG and nasal lavage IgA titers were determined by ELISA. The serum IgG titer induced with (TT)(PLGA) nanospheres was higher than TT solution (P?<?0.001). IgG titers induced with (TT?+?QS)(PLGA) was higher than (TT)(PLGA) (P?<?0.0001). When (TT)(PLGA) and (TT?+?QS)(PLGA) nanospheres were mixed with CDM, higher IgG titers were induced (P?<?0.001). The highest mucosal sIgA titers were seen in animals immunized with (TT?+?QS)(PLGA)?+?CDM. Co-encapsulation of QS and TT in PLGA nanospheres increased sIgA titers. In conclusion, the highest immune responses were observed by concomitant use of three adjuvants.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1097-9867
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
36-43
pubmed:meshHeading
pubmed-meshheading:20082579-Adjuvants, Immunologic, pubmed-meshheading:20082579-Administration, Intranasal, pubmed-meshheading:20082579-Animals, pubmed-meshheading:20082579-Cross-Linking Reagents, pubmed-meshheading:20082579-Dextrans, pubmed-meshheading:20082579-Drug Carriers, pubmed-meshheading:20082579-Drug Stability, pubmed-meshheading:20082579-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:20082579-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:20082579-Immunoglobulin A, pubmed-meshheading:20082579-Immunoglobulin G, pubmed-meshheading:20082579-Lactic Acid, pubmed-meshheading:20082579-Microscopy, Electron, Scanning, pubmed-meshheading:20082579-Microspheres, pubmed-meshheading:20082579-Nanospheres, pubmed-meshheading:20082579-Nasal Mucosa, pubmed-meshheading:20082579-Particle Size, pubmed-meshheading:20082579-Plant Bark, pubmed-meshheading:20082579-Plant Extracts, pubmed-meshheading:20082579-Polyglycolic Acid, pubmed-meshheading:20082579-Quillaja, pubmed-meshheading:20082579-Rabbits, pubmed-meshheading:20082579-Solubility, pubmed-meshheading:20082579-Surface Properties, pubmed-meshheading:20082579-Tetanus Toxoid
pubmed:year
2011
pubmed:articleTitle
Dextran microspheres could enhance immune responses against PLGA nanospheres encapsulated with tetanus toxoid and Quillaja saponins after nasal immunization in rabbit.
pubmed:affiliation
School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't