Linked Life Data

20080449

Source:http://linkedlifedata.com/resource/pubmed/id/20080449

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-5-10
pubmed:abstractText
A new bi-enzymatic system was designed by co-immobilization of alpha-glucosidase (AG) and pyranose oxidase (PyOx) for maltose analysis. The immobilization was carried out by cross-linking enzyme mixture, chitosan (CHIT) and carbon nanotube (CNT) via glutaraldehyde. The structure of biosensor including enzyme, CHIT, glutaraldehyde and CNT amount together with operational conditions like pH, temperature and applied potential were optimized. Then analytical characterization was performed. A fast linear response of the biosensor was observed for maltose in the concentration range from 0.25 to 2.0 mM at 35 degrees C and pH 6.0. The effect of CNT addition into the immobilization matrix was also investigated. The linear relationships between sensor response (y; microA/cm(2)) and substrate concentration (x; mM) were defined by the equations of y=0.844x+0.029 (R(2)=0.999) and y=0.882x+0.0625 (R(2)=0.996) for AG/PyOx/CHIT and AG/PyOx/CHIT-CNT biosensors, respectively. All other data were also given as comparison of two systems one with CNT-modified and CNT-free. Finally, for the sample application, maltose was analyzed in beer samples. As a result, it has been found that; complex matrix of natural beer samples had no influence on the biosensing response. Also the results were in good agreement with those obtained by spectrophotometric measurements.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1878-562X
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2009 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
108-13
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Maltose biosensing based on co-immobilization of alpha-glucosidase and pyranose oxidase.
pubmed:affiliation
Ege University, Faculty of Science, Biochemistry Department, Bornova-Izmir, Turkey.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't