Source:http://linkedlifedata.com/resource/pubmed/id/20079718
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
2010-2-22
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| pubmed:abstractText |
Male rats (21days) were assigned to enriched environment (EE) or to standard environment (SE) for 1year. Oxygen consumption and the sensitivity to calcium induced mitochondrial permeability transition (MPT), through mitochondrial membrane potential (DeltaPsi(m)) and swelling, were determined in isolated hippocampal and cerebral cortex mitochondria. Mitochondrial H(2)O(2) production rate, and NOS activity and expression associated with mitochondrial membranes were also assayed. Results showed that state 3 respiratory rate was increased by 80% in cerebral cortex mitochondria from EE rats and no changes were observed in hippocampal mitochondria after EE exposure. Calcium induced-swelling was 40% and 53% lower in hippocampal and cerebral cortex mitochondria from EE rats, as compared with SE rats. Calcium loading induced membrane depolarization in cerebral cortex mitochondria from EE rats but did not affect mitochondrial DeltaPsi(m) in hippocampal mitochondria from EE animals, probably due to decreased H(2)O(2) formation. NO production associated to mitochondrial membranes was increased by 195% in cerebral cortex mitochondria but decreased by 47% in hippocampal mitochondria from EE rats, as compared with SE rats. Western blot analysis from nNOS protein expression associated to mitochondrial samples revealed a similar pattern. Our results suggest that in hippocampus and cerebral cortex, EE exposure protects mitochondria against calcium-induced MPT maintaining a convenient membrane potential, which assures a continuous energy supply.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type I,
http://linkedlifedata.com/resource/pubmed/chemical/Oxygen
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| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
1872-6240
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2010 Elsevier B.V. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:day |
10
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| pubmed:volume |
1319
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
44-53
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| pubmed:meshHeading |
pubmed-meshheading:20079718-Animals,
pubmed-meshheading:20079718-Calcium,
pubmed-meshheading:20079718-Cerebral Cortex,
pubmed-meshheading:20079718-Environment,
pubmed-meshheading:20079718-Hippocampus,
pubmed-meshheading:20079718-Male,
pubmed-meshheading:20079718-Membrane Potential, Mitochondrial,
pubmed-meshheading:20079718-Mitochondria,
pubmed-meshheading:20079718-Mitochondrial Membranes,
pubmed-meshheading:20079718-Mitochondrial Swelling,
pubmed-meshheading:20079718-Nitric Oxide,
pubmed-meshheading:20079718-Nitric Oxide Synthase,
pubmed-meshheading:20079718-Nitric Oxide Synthase Type I,
pubmed-meshheading:20079718-Oxygen,
pubmed-meshheading:20079718-Random Allocation,
pubmed-meshheading:20079718-Rats,
pubmed-meshheading:20079718-Rats, Sprague-Dawley,
pubmed-meshheading:20079718-Time Factors
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| pubmed:year |
2010
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| pubmed:articleTitle |
Mitochondrial function and nitric oxide production in hippocampus and cerebral cortex of rats exposed to enriched environment.
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| pubmed:affiliation |
Laboratory of Free Radical Biology, School of Pharmacy and Biochemistry, University of Buenos Aires, Argentina. slarnaiz@ffyb.uba.ar
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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