Linked Life Data

20077074

Source:http://linkedlifedata.com/resource/pubmed/id/20077074

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-1-15
pubmed:abstractText
The mitochondrial fatty acid beta-oxidation is integral to normal cellular metabolism and maintenance of cellular energy supplies. Disorders of this pathway interrupt the body's ability to deal with fasting states, as well as compromising the functioning of organs and systems whose high-energy requirements utilize fats for a continuous energy source, such as heart and skeletal muscle. This method quantitatively measures intermediate metabolites of fatty acid beta-oxidation, specifically the 3-hydroxy-fatty acids produced by the third step in the pathway. The method is useful for helping to diagnose disorders of the pathway, especially defects in the L-3-hydroxyacyl CoA dehydrogenases. Serum or plasma samples are used for routine clinical evaluation; however, measurement of 3-hydroxy-fatty acid intermediates in fibroblast cell culture media and in samples from mice also allows the method to be used for research into fatty acid oxidation and interconnected pathways. The method is a stable isotope dilution, electron impact ionization gas chromatography/mass spectrometry (GC/MS) procedure.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1940-6029
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
603
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
229-43
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
3-Hydroxy-fatty acid analysis by gas chromatography-mass spectrometry.
pubmed:affiliation
Department of Pathology, University of Texas Southwestern Medical Center and Children's Medical Center, Dallas, TX, USA.
pubmed:publicationType
Journal Article