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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2010-1-15
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pubmed:abstractText |
A liquid-liquid extraction (LLE) of drugs and internal standard (promazine) is performed by mixing urine at basic pH with 1-chlorobutane. There are no hydrolysis or derivatization steps. After centrifugation the organic (upper) layer is transferred to another tube and evaporated. The dried extract is reconstituted with ethyl acetate and 1 microL is injected onto the GCMS. Drugs are volatilized in the GC inlet and separated on a capillary column. In the EI source drugs become positively charged and fragment. Mass analysis of ionized fragments occurs with a single quadrupole. The resulting full scan mass spectra are automatically searched against three libraries.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1940-6029
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
603
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
187-202
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pubmed:meshHeading |
pubmed-meshheading:20077071-Acetates,
pubmed-meshheading:20077071-Algorithms,
pubmed-meshheading:20077071-Butanes,
pubmed-meshheading:20077071-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:20077071-Humans,
pubmed-meshheading:20077071-Pharmaceutical Preparations,
pubmed-meshheading:20077071-Promazine,
pubmed-meshheading:20077071-Reference Standards,
pubmed-meshheading:20077071-Spectrometry, Mass, Electrospray Ionization
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pubmed:year |
2010
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pubmed:articleTitle |
Broad spectrum drug screening using electron-ionization gas chromatography-mass spectrometry (EI-GCMS).
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pubmed:affiliation |
Clinical Laboratories, San Francisco General Hospital, San Francisco, CA, USA.
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pubmed:publicationType |
Journal Article
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