|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2010-1-15
|
|
pubmed:abstractText |
There is evidence that in the acute axonal motor neuropathy (AMAN) subtype of Guillain-Barré syndrome antibodies to gangliosides, produced through molecular mimicry by antecedent Campylobacter jejuni (C. jejuni) infection, attack gangliosides expressed in human peripheral nerve axolemma, inducing a primary axonal damage. The aim of this study is to investigate whether the T cell response has a role in AMAN pathogenesis. We isolated monocytes from 4 healthy subjects and 5 AMAN patients with antecedent C. jejuni infection and antibodies to GM1 and/or GD1a gangliosides. Immature dendritic cells expressing CD1 molecules cultured with autologous T cells were stimulated with 2 lipopolysaccharides (LPSs) extracted from C. jejuni strains containing GM1 and GD1a-like structures and with GM1 and GD1a. The T cell response to LPSs and to gangliosides was determined by measuring the release of IFN-gamma and TNF-alpha. We observed a T cell response to both LPSs in controls and AMAN patients, whereas only AMAN patients showed T cell reactivity to gangliosides GM1 and GD1a with a tight correlation between T cell reactivity to the ganglioside and individual antibody responses to the same ganglioside. T cells responding to gangliosides were CD1c-restricted CD8 positive and CD27 negative. These findings indicate a contribution of cellular immunity in the pathogenesis of AMAN. A possible role for ganglioside-reactive T cells might be to facilitate the production of antibodies against gangliosides.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD1,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD27,
http://linkedlifedata.com/resource/pubmed/chemical/CD1C protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/G(M1) Ganglioside,
http://linkedlifedata.com/resource/pubmed/chemical/Gangliosides,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/ganglioside, GD1a
|
|
pubmed:status |
MEDLINE
|
|
pubmed:issn |
0394-6320
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
22
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1043-50
|
|
pubmed:meshHeading |
pubmed-meshheading:20074468-Acute Disease,
pubmed-meshheading:20074468-Adult,
pubmed-meshheading:20074468-Aged,
pubmed-meshheading:20074468-Antibodies,
pubmed-meshheading:20074468-Antigens, CD1,
pubmed-meshheading:20074468-Antigens, CD27,
pubmed-meshheading:20074468-Axons,
pubmed-meshheading:20074468-CD8-Positive T-Lymphocytes,
pubmed-meshheading:20074468-Campylobacter Infections,
pubmed-meshheading:20074468-Campylobacter jejuni,
pubmed-meshheading:20074468-Case-Control Studies,
pubmed-meshheading:20074468-Cells, Cultured,
pubmed-meshheading:20074468-Coculture Techniques,
pubmed-meshheading:20074468-Cytotoxicity, Immunologic,
pubmed-meshheading:20074468-Dendritic Cells,
pubmed-meshheading:20074468-Female,
pubmed-meshheading:20074468-G(M1) Ganglioside,
pubmed-meshheading:20074468-Gangliosides,
pubmed-meshheading:20074468-Glycoproteins,
pubmed-meshheading:20074468-Guillain-Barre Syndrome,
pubmed-meshheading:20074468-Humans,
pubmed-meshheading:20074468-Immunity, Cellular,
pubmed-meshheading:20074468-Immunophenotyping,
pubmed-meshheading:20074468-Interferon-gamma,
pubmed-meshheading:20074468-Lipopolysaccharides,
pubmed-meshheading:20074468-Male,
pubmed-meshheading:20074468-Middle Aged,
pubmed-meshheading:20074468-Motor Neuron Disease,
pubmed-meshheading:20074468-Motor Neurons,
pubmed-meshheading:20074468-Tumor Necrosis Factor-alpha,
pubmed-meshheading:20074468-Young Adult
|
|
pubmed:articleTitle |
T cell response in acute motor axonal neuropathy.
|
|
pubmed:affiliation |
Neuroimmunology Unit, European Centre for Brain Research, Santa Lucia Foundation, Rome, Italy.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
