Source:http://linkedlifedata.com/resource/pubmed/id/20067762
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2010-2-17
|
| pubmed:abstractText |
In antioxidant competition assays, an antioxidant (A) and a detector compound (D) compete for a reactive species (R). In the evaluation of these assays, it is tacitly assumed that all of R is captured by either D or A. Due to the - by definition - high reactivity of R, unspecific reactions of R are likely to occur and neglecting these reactions will result in a systematic underestimation of antioxidant activity. It was shown that in the standard hydroxyl radical scavenging assay this was indeed the case; the inaccurate mathematical evaluation resulted in an underestimation of antioxidant activity of 25% in this competition assay. The systematic underestimation of antioxidant activity can be prevented by using an adjusted Stern-Volmer equation that takes into account that only part of R is captured by D or A.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
1090-2104
|
| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:day |
12
|
| pubmed:volume |
392
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
346-50
|
| pubmed:meshHeading | |
| pubmed:year |
2010
|
| pubmed:articleTitle |
Prevention of a systematic underestimation of antioxidant activity in competition assays. The impact of unspecific reactions of the reactive species.
|
| pubmed:affiliation |
Department of Pharmacology and Toxicology, Maastricht University, 6200 MD Maastricht, The Netherlands.
|
| pubmed:publicationType |
Journal Article
|