Linked Life Data

20065502

Source:http://linkedlifedata.com/resource/pubmed/id/20065502

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-1-12
pubmed:abstractText
Although spontaneous phasic activity of detrusor muscle plays an important role in urinary bladder function there is little information regarding myogenic [Ca(2+)](i) signals in this tissue. We have studied spontaneous, unstimulated [Ca(2+)](i) signals in fura-2 loaded detrusor cells isolated from newborn (10-13 days old) guinea-pig urinary bladder. In newborn guinea pigs 35% of studied muscle cells displayed spontaneous [Ca(2+)](i) oscillations with several kinetic patterns (from irregular to highly paced cycles). The oscillations were inhibited by external Ca(2+) removal, treatment with L- and T-type Ca(2+) channel blockers and by the hyperpolarizing drug pinacidil. Ca(2+) stores were necessary to maintain oscillations, as indicated by the inhibitory effects of thapsigargin, ryanodine and 2-APB. Oscillations were also inhibited by folimycin, an inhibitor of acidic Ca(2+) stores. Treatment with the selective inhibitors iberiotoxin and NPPB indicated that the oscillatory signal is also modulated by Ca(2+) -activated K(+) channels (inhibitory) and Ca(2+) -activated Cl(-) channels (stimulatory). Our results indicate that detrusor cells from newborn guinea-pigs develop spontaneous [Ca(2+)](i) oscillations due to Ca(2+) influx through T- and L-type Ca(2+) channels modulated by intracellular stores, including acidic pools. This activity could underlie the myogenic activity of urinary bladder during early stages of development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1899-1505
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
60
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
93-9
pubmed:meshHeading
pubmed-meshheading:20065502-Aging, pubmed-meshheading:20065502-Animals, pubmed-meshheading:20065502-Animals, Newborn, pubmed-meshheading:20065502-Calcium Channels, L-Type, pubmed-meshheading:20065502-Calcium Channels, T-Type, pubmed-meshheading:20065502-Calcium Signaling, pubmed-meshheading:20065502-Fluorescent Dyes, pubmed-meshheading:20065502-Guinea Pigs, pubmed-meshheading:20065502-Inositol 1,4,5-Trisphosphate Receptors, pubmed-meshheading:20065502-Kinetics, pubmed-meshheading:20065502-Membrane Transport Modulators, pubmed-meshheading:20065502-Microscopy, Fluorescence, pubmed-meshheading:20065502-Myocytes, Smooth Muscle, pubmed-meshheading:20065502-Protein Isoforms, pubmed-meshheading:20065502-Ryanodine Receptor Calcium Release Channel, pubmed-meshheading:20065502-Urinary Bladder, pubmed-meshheading:20065502-Urination
pubmed:year
2009
pubmed:articleTitle
Spontaneous calcium oscillations in urinary bladder smooth muscle cells.
pubmed:affiliation
Department of Physiology, Faculty of Veterinary Sciences and Nursing School, University of Extremadura, 10071 Caceres, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't