Linked Life Data

20064466

Source:http://linkedlifedata.com/resource/pubmed/id/20064466

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2010-1-12
pubmed:abstractText
The RNA-binding protein HuD promotes neuronal differentiation by an unknown mechanism. Here we identify an enhancer function of HuD in translation. Translation stimulation by HuD requires both a 3' poly(A) tail and a 5' m(7)G cap structure. We also show that HuD directly interacts with eIF4A. This interaction and the poly(A)-binding activity of HuD are critical for its translational enhancer function because HuD-eIF4A- and HuD-poly(A)-binding mutants fail to stimulate translation. We show that translation of HCV IRES mRNA, which is eIF4A independent, is not stimulated by HuD. We also find that the eIF4A and poly(A)-binding activities of HuD are not only important for stimulating translation but also are essential for HuD-induced neurite outgrowth in PC12 cells. This example of cap-dependent translational regulation might explain at least in part how HuD triggers the induction of neuronal differentiation.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1097-4164
pubmed:author
pubmed:copyrightInfo
2009 Elsevier Inc.
pubmed:issnType
Electronic
pubmed:day
25
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1007-17
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
The ELAV protein HuD stimulates cap-dependent translation in a Poly(A)- and eIF4A-dependent manner.
pubmed:affiliation
Department of Biology, Kobe University, Rokkodaicho, Nada-ku, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't