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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5000
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pubmed:dateCreated |
1991-4-23
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pubmed:databankReference |
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pubmed:abstractText |
A DNA probe that spanned a domain conserved among the proto-oncogene c-rel, the Drosophila morphogen dorsal, and the p50 DNA binding subunit of NF-kappa B was generated from Jurkat T cell complementary DNA with the polymerase chain reaction (PCR) and degenerate oligonucleotides. This probe was used to identify a rel-related complementary DNA that hybridized to a 2.6-kilobase messenger RNA present in human T and B lymphocytes. In vitro transcription and translation of the complementary DNA resulted in the synthesis of a protein with an apparent molecular size of 65 kilodaltons (kD). The translated protein showed weak DNA binding with a specificity for the kappa B binding motif. This protein-DNA complex comigrated with the complex obtained with the purified human p65 NF-kappa B subunit and binding was inhibited by I kappa B-alpha and -beta proteins. In addition, the 65-kD protein associated with the p50 subunit of NF-kappa B and the kappa B probe to form a complex with the same electrophoretic mobility as the NF-kappa B-DNA complex. Therefore the rel-related 65-kD protein may represent the p65 subunit of the active NF-kappa B transcription factor complex.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
251
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pubmed:geneSymbol |
dorsal,
rel
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1490-3
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pubmed:dateRevised |
2007-3-19
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pubmed:meshHeading |
pubmed-meshheading:2006423-Amino Acid Sequence,
pubmed-meshheading:2006423-Blotting, Northern,
pubmed-meshheading:2006423-Cloning, Molecular,
pubmed-meshheading:2006423-DNA,
pubmed-meshheading:2006423-Humans,
pubmed-meshheading:2006423-Macromolecular Substances,
pubmed-meshheading:2006423-Molecular Sequence Data,
pubmed-meshheading:2006423-NF-kappa B,
pubmed-meshheading:2006423-Polymerase Chain Reaction,
pubmed-meshheading:2006423-Proto-Oncogene Proteins,
pubmed-meshheading:2006423-Proto-Oncogene Proteins c-rel,
pubmed-meshheading:2006423-T-Lymphocytes
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pubmed:year |
1991
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pubmed:articleTitle |
Isolation of a rel-related human cDNA that potentially encodes the 65-kD subunit of NF-kappa B.
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pubmed:affiliation |
Department of Molecular Oncology and Virology, Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110-1199.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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