Linked Life Data

20061812

Source:http://linkedlifedata.com/resource/pubmed/id/20061812

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2010-5-20
pubmed:abstractText
AML has a dismal prognosis. It was previously shown that the expression of gene coding for the hyperfusogenic gibbon ape leukemia virus envelope glycoprotein (GALV.fus) can efficiently kill leukemic cells. However, target killing effect of GALV.fus on leukemia cells may be limited. Viral vectors, such as retroviruses and adenoviruses, have been developed to deliver heterologous genes into tumors in vivo, but these vectors have some limitations for gene therapy of leukemia. Another virus that has drawn interest as a gene transfer vector is the Sindbis virus. Sindbis virus efficiently infects human tumor cells through the high-affinity 67 kDa Laminin receptor. We found that Laminin-R was obviously expressed in HL-60 and primary human AML cells, but weakly expressed in K562 cells and blood samples of normal human. So we reasoned that Sindbis-virus-based vectors might be ideal for target gene transfer of GALV.fus to acute myeloid leukemic cell. It was shown that Sindbis virus efficiently transduced human acute myeloid leukemic cells with high expression of Laminin-R and exhibited potent cytopathic potential. What is more, we found that CFU-GMs were significantly reduced after Sindbis virus carrying GALV.fus transduced human primary AML cells. Sindbis virus carrying GALV.fus was active against human AML xenografts in vivo. Taken together, we concluded that Sindbis virus carrying GALV.fus may be an useful strategy for gene therapy of acute myeloid leukemia.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1555-8576
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
350-7
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Gene therapy for acute myeloid leukemia using Sindbis vectors expressing a fusogenic membrane glycoprotein.
pubmed:affiliation
State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-sen University, Guangzhou, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't