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rdf:type |
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lifeskim:mentions |
umls-concept:C0020792,
umls-concept:C0034790,
umls-concept:C0086222,
umls-concept:C0337112,
umls-concept:C0679058,
umls-concept:C1524075,
umls-concept:C1547699,
umls-concept:C1552644,
umls-concept:C1705733,
umls-concept:C1708726,
umls-concept:C1823153,
umls-concept:C2349976,
umls-concept:C2700640
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pubmed:issue |
6
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pubmed:dateCreated |
1991-4-25
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pubmed:databankReference |
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pubmed:abstractText |
The gamma delta and alpha beta T-cell antigen receptor (TCR) heterodimers are expressed in a lineage-specific, mutually exclusive manner. Regulation of expression occurs at the transcriptional level. A 13-kilobase (kb) stretch of DNA encompassing variable-joining-constant segments V gamma 4-J gamma 1-C gamma 1 of the murine gamma-chain gene was examined for the presence of transcriptional enhancing elements by a transient transfection assay. DNA fragments from this region were inserted into a test plasmid containing a heterologous promoter fused to the human growth hormone gene. An 1800-base-pair (bp) fragment located 3 kb 3' to C gamma exon III was found to display enhancing activity in several T-cell lines. Maximum enhancing activity could be localized further to fragments as small as 400 bp in some cell lines. Nucleotide sequence analysis of this 400-bp segment revealed homologies to previously described core enhancer elements and to other TCR gene enhancers. The TCR gamma-chain gene enhancer is active in both gamma delta and alpha beta T cells, indicating that it is not primarily responsible for lineage-specificity of expression, but it is inactive in non-T-cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-2144608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-2154700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-2156339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-2295883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-2326267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-2437578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-2463632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-2524381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-2534795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-2582491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-2784150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-2787028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-2788889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-2943999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-2960972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-2968651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-2983227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-3125549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-3396541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-3871915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-3919308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2006158-6330567
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2204-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2006158-Animals,
pubmed-meshheading:2006158-Base Sequence,
pubmed-meshheading:2006158-Enhancer Elements, Genetic,
pubmed-meshheading:2006158-Macromolecular Substances,
pubmed-meshheading:2006158-Mice,
pubmed-meshheading:2006158-Molecular Sequence Data,
pubmed-meshheading:2006158-Plasmids,
pubmed-meshheading:2006158-Receptors, Antigen, T-Cell,
pubmed-meshheading:2006158-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:2006158-Restriction Mapping,
pubmed-meshheading:2006158-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2006158-T-Lymphocytes
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pubmed:year |
1991
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pubmed:articleTitle |
Identification of a T-cell-specific enhancer at the locus encoding T-cell antigen receptor gamma chain.
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pubmed:affiliation |
Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge 02139.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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